We narrowed to 9,297 results for: yeast expression

1. pCRISPRyl_A08

   Plasmid

   #84610

   Purpose

   Introduces DSB at A08 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPRyl_MFE1

   Plasmid

   #84612

   Purpose

   Introduces DSB at MFE1 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS

   Plasmid

   #52345

   Purpose

   Integration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKate

   Depositor

   Chang Liu

   Article

   Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

   Insert

   mKate2
   

   Use

   P1 integration vector

   Tags

   Expression

   Bacterial

   Mutation

   changed V2H

   Promoter

   pGKL2 ORF10 UCS

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCL-GKL1ShortMutPRC-Ug1Lg2starTGA

   Plasmid

   #52347

   Purpose

   Integration cassette to create p1-Short-UL*(TGA)

   Depositor

   Chang Liu

   Article

   Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

   Insert

   leu*
   

   Use

   P1 integration vector

   Tags

   Expression

   Bacterial

   Mutation

   changed S2F, A3V, Q180*

   Promoter

   UCS from pGKL2 ORF5

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p414GPD_Hsp82viiB

   Plasmid

   #61709

   Purpose

   Yeast plasmid with Hsp82 heterodimer construct.

   Depositor

   Dan Bolon

   Article

   Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

   Insert

   Hsp82viiB (HSP82 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Heteromeric coiled coil inserted after amino acid…

   Promoter

   GPD1

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pESC-LEU-GFP-VHL

   Plasmid

   #21053

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

7. bRA77

   Plasmid

   #100953

   Purpose

   GAL1 driven Cas9. gRNA can be cloned into BplI sites.

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYZ301

   Plasmid

   #102690

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYZ294

   Plasmid

   #102688

   Purpose

   S. pombe gRNA entry vector with Cas9 - KanMX marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYZ300

    Plasmid

    #102689

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp his3 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYZ293

    Plasmid

    #102687

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pESC-URA-GFP-Ubc9wt

    Plasmid

    #20368

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pUC-TALO8

    Plasmid

    #83409

    Purpose

    TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    TRP-ARS-8xLacO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Please see depositor comments below

    Promoter

    TRP1

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MCV

    Plasmid

    #81174

    Purpose

    MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. p109.1

    Plasmid

    #44518

    Depositor

    Michael Grunstein

    Article

    Hecht et al Cell. 1995 Feb 24;80(4):583-92.

    Insert

    Histone 4
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains full length (aa 1-102)

    Promoter

    Available Since

    May 21, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pESC-URA-Ub-G76A-Rnq1-GFP

    Plasmid

    #21052

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP and ubiquitin

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pAG413 GAL ORF1 Shuffle1 NLS

    Plasmid

    #145794

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Payero et al Journal of the South Carolina Academy of

    Insert

    ORF1 Shuffle1 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Enhanced Nucleur Localization Signal

    Promoter

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDZ207 (loxP KAN loxP 24xPP7SL MDN1)

    Plasmid

    #35191

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    loxP KAN loxP 24xPP7SL MDN1
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP8A-ccdB

    Plasmid

    #83883

    Purpose

    Destination vector for integration at HIS3, expression from the ADH1 promoter

    Depositor

    Jennifer Nemhauser

    Article

    Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS305-mAID-LaM8

    Plasmid

    #198415

    Purpose

    Expresesses mAID-Nb(LaM8) under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    mAID-Nb (LaM8)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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