We narrowed to 13,748 results for: TIM;

1. ICK gRNA (BRDN0001146903)

   Plasmid

   #77414

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ICK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ICK
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. P21-sp64 Poly A

   Plasmid

   #59433

   Purpose

   synthetic poly A transcripts of VZV

   Depositor

   Randall Cohrs

   Article

   Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

   Insert

   VZV ORF 21
   

   Use

   Makes synthetic transcripts that contains poly a

   Tags

   none

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. SPHK2 gRNA (BRDN0001146882)

   Plasmid

   #77096

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. SH3BP4 gRNA (BRDN0001149529)

   Plasmid

   #78087

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SH3BP4

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SH3BP4 (SH3BP4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SH3BP4 gRNA (BRDN0001487091)

   Plasmid

   #78089

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SH3BP4

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SH3BP4 (SH3BP4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p300dAIL KAT LIC 1M

   Plasmid

   #233588

   Purpose

   Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300dAIL KAT (EP300 Human)
   

   Use

   Tags

   6xHis MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 1523-1554

   Promoter

   T7

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

   Plasmid

   #230075

   Purpose

   Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

   Depositor

   Sumeet Pal Singh

   Article

   Hovhannisyan et al bioRxiv 2025.05.23.655316

   Insert

   lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   fabp10a

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas3-CDA1

   Plasmid

   #229534

   Purpose

   pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   Cas3
   Cytidine deaminase
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROP-seq-copGFP-v2-no-scaffold

   Plasmid

   #228520

   Purpose

   CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

   Depositor

   Jellert Gaublomme

   Article

   Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CROP-seq-puro-v2-no-scaffold

    Plasmid

    #228521

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCA528 IST1 (1-366)

    Plasmid

    #193039

    Purpose

    Bacterial expression plasmid for full-length IST1 (1-366).

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA528 CAPN7 (1-75)

    Plasmid

    #193031

    Purpose

    Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 1-75

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CAPN7 (81-165)

    Plasmid

    #193032

    Purpose

    Bacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domain

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 81-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CROP-seq-mTurquoise2-v2-no-scaffold

    Plasmid

    #228522

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABEmax-Puro V2

    Plasmid

    #226955

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC-hU6-crBACH1-array_EF1a-BFP

    Plasmid

    #224787

    Purpose

    BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBACH1-1, crBACH1-2, crBACH1-3,
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUt-mNF-Cas13d

    Plasmid

    #224789

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LPUtopia-7_CoV-2

    Plasmid

    #224782

    Purpose

    Landing Pad donor vector containing partial RdRp and N gene sequences from SARS-CoV-2 for fluorescent reporter assay

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    partial RdRp and N gene (N, ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid

    #169135

    Purpose

    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Coh7
    

    Use

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28:LanIV-Chondromyces

    Plasmid

    #225078

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Chondromyces)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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