We narrowed to 2,346 results for: puc19

1. pTR-374 tNGFR-P2A-B2M

   Plasmid

   #186068

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   B2M (B2M Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTR-471 IRF4-tNGFR

   Plasmid

   #186061

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   IRF4 (IRF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT1219

   Plasmid

   #228301

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Severe acute respiratory syndrome-related coronavirus 2, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   GGG linker followed by His6 and MF_ secretion sig…

   Expression

   Yeast

   Mutation

   G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIG-749_HA-GD2-28z_CAR_TFAP4

   Plasmid

   #207490

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

   Plasmid

   #207493

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIG-851_HA-GD2-28z_HA-TFAP4

   Plasmid

   #207501

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIG-852_HA-GD2-28z_TFAP4-HA

   Plasmid

   #207502

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIG-719 CTLA4-GFP R70W

   Plasmid

   #186124

   Purpose

   CTLA4 gene knockin with mutation

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   R70W

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIG-720 CTLA4-GFP R75W

   Plasmid

   #186125

   Purpose

   CTLA4 gene knockin with mutation

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   R75W

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTR-375 tNGFR-P2A-ITGB7

    Plasmid

    #186069

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    ITGB7 (ITGB7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge