We narrowed to 18,031 results for: Mut

1. pSerOTS-C1* (V70)

   Plasmid

   #188537

   Purpose

   Phosphoserine incorporation into recombinant proteins

   Depositor

   Jesse Rinehart

   Article

   Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

   Inserts

   pSerRS
   Ef-pSer
   tRNApSer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations resulting from laboratory evolution

   Promoter

   glnS*

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. VRQR-ABEmax

   Plasmid

   #119811

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ArcLight-A242

   Plasmid

   #36857

   Depositor

   Vincent Pieribone

   Article

   Jin et al Neuron. 2012 Sep 6;75(5):779-85.

   Insert

   fluorescent protein voltage sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   semian CMV

   Available Since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICH86966::AtU6p::sgRNA_PDS

   Plasmid

   #46966

   Purpose

   Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p::sgRNA_PDS
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-EGxxFP

   Plasmid

   #50716

   Purpose

   5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Insert

   split EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. hGFAP-NLS-HA-Dre-P2A-BFP

   Plasmid

   #51277

   Purpose

   Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-Dre-P2A-BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hGFAP

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCiPS-NIFV

   Plasmid

   #110807

   Purpose

   Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells

   Depositor

   David Russell

   Article

   Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

   Insert

   Pol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 mutations in the DDE catalyic core motif of the…

   Promoter

   CMV

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPTN-EGFP

   Plasmid

   #27052

   Depositor

   Beatrice Yue

   Article

   Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3xFLAG-TYR

   Plasmid

   #32780

   Depositor

   Ruth Halaban

   Article

   Halaban et al J Invest Dermatol. 2002 Aug;119(2):481-8

   Insert

   Tyrosinase (TYR Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pGGF_RT_FAST_RFP

    Plasmid

    #136979

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Article

    Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

    Insert

    seed coat marker RFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Promoter

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE508

    Plasmid

    #153280

    Purpose

    End-linker for cloning 2 sgRNA arrays into any recipient vector

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    linker
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORTMAGE-Pa1

    Plasmid

    #138475

    Purpose

    Enables high-efficiency recombineering in P. aeruginosa

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    PapRecT
    mutL E36K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E36K

    Promoter

    Available Since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. HBV 1.3-mer X-null replicon

    Plasmid

    #65461

    Purpose

    containing 1.3 units of the X-null HBV genome (subtype ayw)

    Depositor

    Wang-Shick Ryu

    Article

    Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

    Insert

    1.3 units of the X-null HBV genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-FLAG

    Plasmid

    #183511

    Purpose

    Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. epi-ABEmax-NG

    Plasmid

    #135976

    Purpose

    Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pTara:500

    Plasmid

    #60717

    Purpose

    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-neo Fluc EGFP

    Plasmid

    #90170

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Keratin 8 (pcDNA3)

    Plasmid

    #18063

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. HDM_VSV_G

    Plasmid

    #204156

    Purpose

    VSV-G expression plasmid

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    VSV-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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