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pBBR1k-con-GFPuv

Plasmid

#106386

Purpose

Constitutive trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFP-KASH

Plasmid

#154373

Purpose

Vector for Cre-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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rAAV2-retro helper

Plasmid

#81070

Purpose

retrograde AAV helper vector, carrying rep and modified capsid gene

Depositor

Alla Karpova, David Schaffer

Article

Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

Inserts

rep
rAAV2-retro cap

Use

AAV and Unspecified; Helper vector

Tags

Expression

Mutation

Pro2Ala, possibly Asp613Gly and changes made to A…

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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phCMV-GALV-MTR

Plasmid

#163612

Purpose

Retro and Lentiviral transduction of primary human germinal center B cells

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

GaLV MTR envelope

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-VPR

Plasmid

#63800

Purpose

SP-dCas9-VPR with doxycycline-inducible expression

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-sadCas9-VP64

Plasmid

#115790

Purpose

An AAV vector expressing S. aureus dCas9 fused to VP64.

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

Sa-dCas9

Use

AAV

Tags

NLS and NLS-VP64

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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STING-V2

Plasmid

#124264

Purpose

C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

Depositor

Eric Schirmer

Insert

TMEM173 (STING1 Human)

Use

Tags

Venus2

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1

Plasmid

#155346

Purpose

Express full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (YTHDF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET30b-7+-SrtA

Plasmid

#105602

Purpose

expressed 7+ sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7+ mutant

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGreenFire1_E2F1RE

Plasmid

#112248

Purpose

expression of GFP and Luciferase under the control of E2F1 responsive element

Depositor

Simone Di Giovanni

Insert

E2F1RE (responsive element)

Use

Lentiviral

Tags

copGFP and luciferase

Expression

Mutation

Promoter

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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