We narrowed to 38,210 results for: NAM

1. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

   Plasmid

   #107729

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/BirA/FLAG-DDX54

   Plasmid

   #97063

   Purpose

   Expresses BirA/FLAG-tagged DDX54 in mammalian cells

   Depositor

   Markus Landthaler

   Article

   Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

   Insert

   DDX54 (DDX54 Human)
   

   Tags

   BirA and FLAG

   Expression

   Mammalian

   Promoter

   tetO2

   Available Since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-SV40P-Tet1 enhancer E

   Plasmid

   #63880

   Purpose

   Luciferase reporter plasmid used to study transcriptional control of murine Tet1

   Depositor

   Kian Peng Koh

   Article

   Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

   Insert

   Tet1 enhancer fragment E
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

   Plasmid

   #179428

   Purpose

   Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-(nospacer)-mch
   

   Expression

   Mammalian

   Promoter

   pEF alpha, pRSV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA BRCA1 Q1779Stop

   Plasmid

   #139331

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 Q1779Stop using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLR5-CAG-NLS-tdiRFP670-2A-LBR

   Plasmid

   #82620

   Purpose

   Expresses NLS-tdiRFP-2A-LBR in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-DN-RFX

   Plasmid

   #52296

   Purpose

   Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   Dominant negative RFX
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA BRCA1 T922I

   Plasmid

   #139328

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 T922I using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 T922I using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB-hCRY1-SNAP-CD4-bla

   Plasmid

   #179447

   Purpose

   Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   SnapTag
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-CCre-MBP1-WPRE

    Plasmid

    #193917

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP1
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-NCre-MBP6-WPRE

    Plasmid

    #193916

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    NCre-MBP6
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. nuc-yHS1-M7A,H102A

    Plasmid

    #159168

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A,H102A
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mito-yHS1-M7A,H102A

    Plasmid

    #159165

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A,H102A
    

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid

    #119864

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    HA-tagged biotin ligase (BirA), Thosea asigna vir…

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-BASIC- ARHGAP29 Mutant PROMOTER

    Plasmid

    #104156

    Purpose

    luciferase reporter with ARHGAP29 mutant promoter

    Depositor

    Marius Sudol

    Article

    Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

    Insert

    ARHGAP29 promoter (ARHGAP29 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    delete two TEAD binding sites

    Promoter

    ARHGAP29

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS DV DNM1-EGFP

    Plasmid

    #107794

    Purpose

    donor vector for knock-in gene editing at human DNM1 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    DNM1-eGFP (DNM1 Synthetic, Human)
    

    Use

    Yeast-e.coli shuttle vector

    Expression

    Bacterial and Yeast

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-Bin1b

    Plasmid

    #176014

    Purpose

    Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. B52 + TIMELESS sgSTOP

    Plasmid

    #100713

    Purpose

    B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting TIMELESS (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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