Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 60,369 results for: PLE;

Showing: 4561 - 4580 of 60369 results

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myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182854

Purpose

Hetero-tetrmer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mCherry2-mEGFP-mApple

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166133

Purpose

This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting cldn1 gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 P290L BLAST

Plasmid

#164239

Purpose

lentiviral plasmid for GFP-TREX1 P290L

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

P290L point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 Y305C BLAST

Plasmid

#164240

Purpose

lentiviral plasmid for GFP-TREX1 Y305C

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Y305C point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 D272fs BLAST

Plasmid

#164237

Purpose

lentiviral plasmid for GFP-TREX1 D272fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D272 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 L287fs BLAST

Plasmid

#164238

Purpose

lentiviral plasmid for GFP-TREX1 L287fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

L287 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-puro

Plasmid

#156169

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 309* BLAST

Plasmid

#164242

Purpose

lentiviral plasmid for GFP-TREX1 309*

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 310-314

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 G306A BLAST

Plasmid

#164241

Purpose

lentiviral plasmid for GFP-TREX1 G306A

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

G306A point mutation

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG

Plasmid

#130680

Purpose

Expresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_G1960del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG

Plasmid

#130677

Purpose

Expresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

M1961_E1992del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG

Plasmid

#130689

Purpose

Expresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, M1961_E1992del

Promoter

CMV

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP(1-233)-mApple(2-236)-HRas(2-189)

Plasmid

#133855

Purpose

Mammalian expression of HRas N-terminally tagged with EGFP and mApple

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

HRas (HRAS Human)

Tags

EGFP and mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG

Plasmid

#130686

Purpose

Expresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-9.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I378_G1960del

Promoter

CMV

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG

Plasmid

#130685

Purpose

Expresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-9.41-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I378_Q1684del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG

Plasmid

#130682

Purpose

Expresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

E653_G1960del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAG

Plasmid

#130687

Purpose

Expresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-9.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I378_E1992del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti C-MYC-DDK Puro PNRC1-W300A

Plasmid

#123301

Purpose

Mammalian expression of PNRC1-W300A mutant C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Mutation

W300A

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/T35A-IRES-Puro

Plasmid

#120571

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/Y40A-IRES-Puro

Plasmid

#120573

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 4561 - 4580 of 60369 results

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