We narrowed to 1,293 results for: nps

1. UbG76V-eGFP-V5His_20aa

   Plasmid

   #23970

   Depositor

   Nico Dantuma

   Article

   Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

   Insert

   Ub-G76V-eGFP-V5HIS_20aa
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   G76V mutation in Ubiquitin. Ub fused to N-termin…

   Promoter

   Available since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. vas-Cas9.874Z

   Plasmid

   #112687

   Purpose

   Express hSpCas9 under vasa promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   vasa

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ub[9]-mRFP[1]

   Plasmid

   #20249

   Depositor

   Nico Dantuma

   Article

   Salomons et al Mol Cell Biol. 2009 Jan 21. ():.

   Insert

   ubiquitin C (UBC Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   changed Stop codon 686 to Glycine

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMD19-PBBa_J23117-sgRNA-Spr

   Plasmid

   #190793

   Purpose

   Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA (dummy)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23117

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-EPN-01(ΔL1)

   Plasmid

   #80049

   Purpose

   Expression of EPN-01 mutated in TSG101 binding site.

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN-01(deltaPTAP)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   PTAP10AAAA

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159672

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   βTub-NLS-hSpCas9-T2A-GFP
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159671

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   ExuL-NLS-hSpCas9-T2A-GFP
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-EPN-01*(ΔM)

   Plasmid

   #80040

   Purpose

   Expression or G2A mutant of EPN-01*

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN-01*(G2A)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Gly2 mutated to Ala

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

   Plasmid

   #131618

   Purpose

   Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR[dsxM]
   NeoR[traF]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Opie2

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA[bTub].1026C

    Plasmid

    #112691

    Purpose

    express gRNA targeting bTub under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    dU6-3

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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