Addgene

We narrowed to 4,413 results for: Ado;

Showing: 661 - 690 of 4413 results

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pUNIV-EGFP

Plasmid

#24706

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Insert

EGFP (eGFP Aequoria victoria)

Use

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn3beta AS4(-)

Plasmid

#58269

Purpose

Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn3beta AS4(-) (Nrxn3 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-EVE-MM

Plasmid

#22994

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A, S463E, S465E

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-GM

Plasmid

#22992

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S210A ,T202A, S191A, S198A

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pOPINK-UBE2S

Plasmid

#66714

Purpose

Expresses UBE2S (1-196) in E. coli

Depositor

David Komander

Article

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Insert

e2-epf (UBE2S )

Use

Tags

GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pFPL-Rg

Plasmid

#61654

Purpose

Gateway vector for generating red fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-VPS35

Plasmid

#166220

Purpose

Gateway entry vector for N. furzeri VPS35

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al bioRxiv

Insert

VPS35

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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Rtn3c-mEmerald

Plasmid

#186963

Purpose

Express Rtn3c with C-terminus mEmerald

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

RTN3C (Rtn3 Mouse)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFPL-Ch

Plasmid

#61647

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFPL-Gh

Plasmid

#61648

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pFPNL1

Plasmid

#61651

Purpose

Gateway vector for generating nucleus-targeted fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-HA-rat Nrxn1beta AS4(-)

Plasmid

#58267

Purpose

Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1beta AS4(-) (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJS124

Plasmid

#207593

Purpose

GFP-BIB H20A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BIB H20A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-EPSILON

Plasmid

#53276

Purpose

Contains ctrE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and the lcyE gene of Lactuca sativa and thereby produces epsilon-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

lcyE

Use

Low copy number bacterial cloning vector

Tags

Expression

Mutation

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn2beta AS4(-)

Plasmid

#58268

Purpose

Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn2beta AS4(-) (Nrxn2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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GatA_pET29b

Plasmid

#215406

Purpose

Expression construct for GatA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

GatA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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CMV566 A-USF

Plasmid

#33360

Depositor

Charles Vinson

Article

Qyang et al Mol Cell Biol. 1999 Feb;19(2):1508-17.

Insert

USF (Usf1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Dominant Negative (see notes below)

Promoter

CMV

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pJS96

Plasmid

#207588

Purpose

GFP-TonBH20A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonBH20A

Use

Tags

Expression

Bacterial

Mutation

H20A

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAC155-pCR8-sgExpression

Plasmid

#49045

Purpose

U6-driven sgRNA expressing cassette on a gateway donor vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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PRExpress-Bxb1-hsp70pA

Plasmid

#158391

Purpose

heat shock inducible Bxb1 integrase for drosophila melanogaster

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

Bxb1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 pET15b

Plasmid

#60677

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26I DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26I

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
NCVB-ssGrl1-ESCargo*

Plasmid

#140167

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophila

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssGrl1-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Tags

Expression

Mutation

Promoter

MTT1

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_ONECUT2

Plasmid

#135738

Purpose

Donor vector for 3' FLAG tag of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 Homology arms (ONECUT2 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PH-GAP1IP4BP

Plasmid

#20200

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 19. ():.

Insert

PH-GAP1IP4BP (RASA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Isolated PH/Btk domain

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pLNCX2-lifeact-mAPPLE

Plasmid

#89820

Purpose

mAPPLE labeled fluorescent actin

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

lifeact-mAPPLE

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII p16

Plasmid

#16455

Depositor

Bert Vogelstein

Article

Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

Insert

p16 (CDKN2A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
UAS-Ceru-10unit

Plasmid

#158389

Purpose

10-unit intMEMOIR recording array for Drosophila melanogaster

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

10unit recording array

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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A104T DJ-1 pET15b

Plasmid

#60680

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

A104T DJ-1 pET15b (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A104T

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pNVL-pvdIJD

Plasmid

#169872

Purpose

Integration vector for deleting pvdIJD locus in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge