-
Plasmid#72952PurposeLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - C site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-lacZ
Plasmid#72953PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-B-RFP
Plasmid#72955PurposeLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - B site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-C-RFP
Plasmid#72956PurposeLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - C site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-RFP
Plasmid#72957PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-b-RFP
Plasmid#72959PurposeLevel 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABCDepositorInsertLevel 2 - b site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCEB3B
Plasmid#67117PurposeStandard for cell-free expression benchmarking.DepositorInsertTCEB3B (ELOA2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 ZFYVE9
Plasmid#67134PurposeStandard for cell-free expression benchmarking.DepositorInsertZFYVE9 (ZFYVE9 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationF1359IPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Short P2A
Plasmid#213614PurposeDetect the short-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Long P2A
Plasmid#213613PurposeDetect the long-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SB100X in pCAG globin pA
Plasmid#127909PurposeSleeping Beauty Transposase expression vector driven by the CAG promoterDepositorInsertSleeping Beauty Transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-GFP
Plasmid#78548PurposeCas9 protein with GFPDepositorInsertT2A-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-BFP
Plasmid#78545PurposeFusion protein Cas9+BFPDepositorInsertBFP
UseLentiviralTagsExpressionMammalianMutationPromoterFusion protein with Cas9Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hFrataxin-HA
Plasmid#31895DepositorInsertFrataxin (FXN Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-BFP
Plasmid#78547PurposeCas9 protein with BFPDepositorInsertT2A-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-GFP
Plasmid#78546PurposeFusion protein Cas9+GFPDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterFusion protein with Cas9Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB2
Plasmid#162690PurposeExpresses DabAB2 inorganic carbon transport complexDepositorInsertDabAB2 inorganic carbon transport complex
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (p15A origin)
Plasmid#74088PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorInsertIL6 (IL6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_IRES_tdTomato
Plasmid#184046PurposeExpresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequenceDepositorInsertsEnhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GDI2
Plasmid#67093PurposeStandard for cell-free expression benchmarking.DepositorInsertGDI2 (GDI2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (p15A origin)
Plasmid#74090PurposepTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterR0010 Const. Promoter controlling RFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CALR
Plasmid#67086PurposeStandard for cell-free expression benchmarking.DepositorInsertCALR (CALR Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL1B
Plasmid#67066PurposeStandard for cell-free expression benchmarking.DepositorInsertIL1B (IL1B Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterR0010 Const. Promoter controlling RFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (colE1 origin)
Plasmid#74091PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TGFB1
Plasmid#67080PurposeStandard for cell-free expression benchmarking.DepositorInsertTGFB1 (TGFB1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CIRBP
Plasmid#67055PurposeStandard for cell-free expression benchmarking.DepositorInsertCIRBP (CIRBP Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS+His6+thrombin
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 ATF1
Plasmid#67065PurposeStandard for cell-free expression benchmarking.DepositorInsertATF1 (ATF1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 ANXA1
Plasmid#67071PurposeStandard for cell-free expression benchmarking.DepositorInsertANXA1 (ANXA1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (colE1 origin)
Plasmid#74092PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT3
Plasmid#67120PurposeStandard for cell-free expression benchmarking.DepositorInsertSTAT3 (STAT3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 ISG15
Plasmid#67054PurposeStandard for cell-free expression benchmarking.DepositorInsertISG15 (ISG15 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GATA1
Plasmid#67068PurposeStandard for cell-free expression benchmarking.DepositorInsertGATA1 (GATA1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 HDAC1
Plasmid#67101PurposeStandard for cell-free expression benchmarking.DepositorInsertHDAC1 (HDAC1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5A0-T
Plasmid#92016PurposeExpresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterJ23102 Promoter (from Anderson promoter library) …Available sinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CTTN
Plasmid#67100PurposeStandard for cell-free expression benchmarking.DepositorInsertCTTN (CTTN Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only