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pU6-crRNA(SOD1)

Plasmid

#109321

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (SOD1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSUM-GroEL-gfp

Plasmid

#109286

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GroEL-GFP

Use

Tags

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

GroEL

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAZ_G-alpha

Plasmid

#109949

Purpose

Protein expression and purification of GNAZ_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAZ_G-alpha (GNAZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPAG1_TPR

Plasmid

#109873

Purpose

Protein expression and purification of SPAG1_TPR

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPAG1_TPR (SPAG1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR0B1_Hormone-recep

Plasmid

#109835

Purpose

Protein expression and purification of NR0B1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR0B1_Hormone-recep (NR0B1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSum-LacZ-gfp

Plasmid

#109285

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

LacZalpha

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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830 pcDNA3 GFP PTEN 1-336

Plasmid

#10927

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Amino acids 1-336

Promoter

Available since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only
pHH0103_TLN1_IRS

Plasmid

#109938

Purpose

Protein expression and purification of TLN1_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TLN1_IRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hisA cdc25c C330S C377S

Plasmid

#10965

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S C377S (CDC25C Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

C330S C377S. Cysteine to Serine substitution at …

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pstop

Plasmid

#109066

Purpose

inserts a polyA site to end transcription of a targetted gene

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NXT1_NTF2

Plasmid

#109803

Purpose

Protein expression and purification of NXT1_NTF2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NXT1_NTF2 (NXT1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab3aa

Plasmid

#109703

Purpose

Multisite gateway vector for 3' tagging with rab3aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3aa (rab3aa Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_FRS2_IRS

Plasmid

#109815

Purpose

Protein expression and purification of FRS2_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

FRS2_IRS (FRS2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAb

Plasmid

#109579

Purpose

Multisite gateway vector for 3' tagging with RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroME(F243Y)-mRuby2-ST

Plasmid

#109127

Purpose

Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME(F243Y)-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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1241 pSG5L HA PTEN G129R A4

Plasmid

#10948

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

G129R; A4: S380A, T382A, T383A, S385A

Promoter

Available since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only
pEGFPN1zetaYF1-6

Plasmid

#109295

Purpose

Express TCR zeta-GFP fusion protein

Depositor

Nicolai van Oers

Insert

TCR zeta (Cd247 Mouse)

Use

Tags

Expression

Mammalian

Mutation

All six Y to F

Promoter

CMV

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR5A1_Hormone-recep

Plasmid

#109792

Purpose

Protein expression and purification of NR5A1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR5A1_Hormone-recep (NR5A1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CBX5_Chromo-shadow

Plasmid

#109805

Purpose

Protein expression and purification of CBX5_Chromo-shadow

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CBX5_Chromo-shadow (CBX5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRS415-BUD32

Plasmid

#10991

Depositor

Francisco Malagon

Insert

BUD32 (BUD32 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Wild type

Promoter

Available since

Dec. 29, 2005

Availability

Academic Institutions and Nonprofits only

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