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Plasmid#225936PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signalDepositorInsertmScarlet3-LactC2
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-ARL13B
Plasmid#227276PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.DepositorInsertsgRNA Targeting C-terminus of ARL13B (ARL13B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mScarletI
Plasmid#221157PurposeFluorescent reporter for cytoplasmic aggregatesDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmScarletIExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mScarlet-I(+5)
Plasmid#219418PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmScarlet-I(+5)
UseTagsMGHHHHHGGExpressionBacterialMutationD125K, D186K, E216K, E238KPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaRL
Plasmid#216903PurposeMega' with knockout of GvpR and GvpLDepositorInsertMega' with knockout of GvpR and GvpL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaRLJ
Plasmid#216956PurposeMega' with knockout of GvpR, GvpL and GvpJDepositorInsertMega' with knockout of GvpR, GvpL and GvpJ
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ARL16-His6
Plasmid#216484PurposeUsed for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tagDepositorInsertADP-ribosylation factor like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mito-CCM2-mScarlet-I
Plasmid#208886PurposeExpress mScarlet-I-CCM2 targeted to the mitochondria in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagsmScarlet-IExpressionMammalianMutationCCM2 targeted to the mitonchondriaPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-PDCD10-mScarlet-I
Plasmid#208887PurposeExpress mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.DepositorInsertCCM3 (PDCD10 Human)
UseTagsmScarlet-IExpressionMammalianMutationPDCD10 targeted to the mitonchondriaPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CKII(0.4)_AzPPO_D14_ts_mScarlet_Kv2.1
Plasmid#205288PurposeBistable opsin for neuronal inhibitionDepositorInsertParapinopsin
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GpACR1-pcDNA3.1-mScarlet
Plasmid#204964PurposeExpression of GpACR1 in fusion with mScarlet in mammalian cellsDepositorInsertGpACR1
UseTagsmScarletExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-hARL1(Q71L)
Plasmid#206407PurposeMammalian expression of hARL1(Q71L)DepositorInsertARL1 (ARL1 Human)
UseTagsEGFPExpressionMammalianMutationQ71LPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-hARL1(T31N)
Plasmid#206408PurposeMammalian expression of hARL1(T31N)DepositorInsertARL1 (ARL1 Human)
UseTagsEGFPExpressionMammalianMutationT31NPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTq2-ymScarletI
Plasmid#205512PurposeExpression of ymTq2-ymScarletl in yeastDepositorInsertymTq2-ymScarletl
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.3
Plasmid#206137PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1 (Col1a1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL5B-His6
Plasmid#201870PurposeUsed for bacterial expression of human protein ARL5BDepositorInsertARL5B
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL1-His6 (CO)
Plasmid#201866PurposeUsed for bacterial expression of human protein ARL1DepositorInsertARL1 (ARL1 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-[Δ1-32]ARL10-His6(CO)
Plasmid#201876PurposeUsed for bacterial expression of human protein ARL10DepositorInsertARL10 (ARL10 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationDeletion of residues 1-32 from N-terminus and cod…PromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL14-His6
Plasmid#201878PurposeUsed for bacterial expression of human protein ARL14DepositorInsertARL14 (ARL14 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL8A-His6
Plasmid#201872PurposeUsed for bacterial expression of human protein ARL8ADepositorInsertARL8A (ARL8A Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only