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pJK210

Plasmid

#73325

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK265

Plasmid

#73598

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioC

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK261

Plasmid

#73323

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioA with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK196

Plasmid

#73322

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK266

Plasmid

#73326

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK195

Plasmid

#73321

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioC

Tags

His6

Expression

Bacterial

Mutation

contains silent mutation in Val428

Promoter

T7

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-RsACE2

Plasmid

#159665

Purpose

Expresses Rhinolophus sinicus ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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A1-LCD Aro+

Plasmid

#169717

Purpose

Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJK234

Plasmid

#73324

Purpose

Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC

Depositor

T. J. Kappock

Inserts

violacein biosynthesis protein VioA
violacein biosynthesis protein VioB
violacein biosynthesis protein VioC

Expression

Bacterial

Promoter

T7 and n/a

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK021

Plasmid

#75060

Purpose

Produces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)

Depositor

T. J. Kappock, JoAnne Stubbe

Insert

phosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)

Expression

Bacterial

Mutation

E. coli optimized codons in first 25 residues

Promoter

lac

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA

Plasmid

#48884

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Danino et al Nature. 2010 Jan 21;463(7279):326-30. do

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pJEC637

Plasmid

#182956

Purpose

AD-SYNZIP: αNTD (P. aeruginosa) – SYNZIP17

Depositor

James Chappell

Article

Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

Insert

AlphaNTD( P. aeruginosa) - SynZip17

Tags

SynZip17

Expression

Bacterial

Promoter

J23106

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJK210

Plasmid

#73325

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK265

Plasmid

#73598

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioC

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK261

Plasmid

#73323

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioA with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK196

Plasmid

#73322

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b-PaNAS

Plasmid

#110801

Purpose

Expresses PaNAS nicotianamine synthase from pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4836

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b- pchB WT

Plasmid

#70099

Purpose

expresses isochorismate-pyruvate lyase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Zaitseva et al J Biol Chem. 2006 Nov 3;281(44):33441-9.

Insert

pchB

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
CoV2-Spike-D614G-Beta

Plasmid

#177962

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )

Expression

Mammalian

Mutation

K417N, E484K, N501Y, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Epsilon

Plasmid

#177964

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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