Skip to main content
Addgene
Showing: 441 - 460 of 17483 results

  1. RRRAA-5-mut-pHG165c

    Plasmid
    #170113
    Purpose
    Constitutively expressed, mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.
    Depositor
    Liskin Swint-Kruse
    Article
    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.
    Insert
    RRRAA-5-MUT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    5A14L, W116Q, E178K, S184T, S237L
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170113-plasmid-map-sequence-id-333275

    Enlarge

  2. SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut

    Plasmid
    #169298
    Purpose
    Constitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    Arid3a-3'UTR-Mut (Arid3a Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169298-plasmid-map-sequence-id-337864

    Enlarge

  3. SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut

    Plasmid
    #169296
    Purpose
    Constitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    ARID3A-3'UTR-Mut (ARID3A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169296-plasmid-map-sequence-id-337837

    Enlarge

  7. pEGFP-rUSP19CYT-CAmut

    Plasmid
    #155250
    Purpose
    for mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155250-plasmid-map-sequence-id-311949

    Enlarge

  8. pEGFP-rUSP19ER-CAmut

    Plasmid
    #155249
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CA mutant (Usp19 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155249-plasmid-map-sequence-id-311947

    Enlarge

  9. pGL3-Basic-ZC3H12D_PromoterMUT

    Plasmid
    #153067
    Purpose
    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter
    Depositor
    Silvia Monticelli
    Article
    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi
    Insert
    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutagenesis to disrupt 3 BHLHE4040 binding sites.…
    Promoter
    None
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    153067-plasmid-map-sequence-id-304643

    Enlarge

  10. pcDNA3-CBX1mut-Dronpa

    Plasmid
    #138250
    Purpose
    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
    Depositor
    Jin Zhang
    Article
    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:
    Insert
    CBX1mut-Dronpa
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138250-plasmid-map-sequence-id-307434

    Enlarge

Showing: 441 - 460 of 17483 results