We narrowed to 19,575 results for: bon

1. pJK040 (pET-PurQ-A128T)

   Plasmid

   #73840

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A128T mutant

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK598

   Plasmid

   #72523

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK612

   Plasmid

   #72533

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK613

   Plasmid

   #72534

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK619

   Plasmid

   #72536

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTRIP-SFFV-mtagBFP-2A

   Plasmid

   #102585

   Purpose

   Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJB158

   Plasmid

   #202601

   Purpose

   RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

   Depositor

   Athanasios Typas

   Article

   Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAN147

    Plasmid

    #129202

    Purpose

    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TR-Csy4-H29A

    Plasmid

    #80602

    Purpose

    Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-H29A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    His29 mutated to Ala

    Promoter

    CBA

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p3A9cbetaTCR.HSV2.3

    Plasmid

    #69594

    Purpose

    TCR beta chain genomic transgenic construct for generation of gBT-I mice

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR beta VJ region from clone HSV2.3 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pJK130

    Plasmid

    #71129

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed valine-319 to phenylalanine

    Promoter

    T7

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK173

    Plasmid

    #71831

    Purpose

    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genes

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK174

    Plasmid

    #71835

    Purpose

    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7 and endogenous

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK175

    Plasmid

    #71132

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKS913.CB18.31

    Plasmid

    #69569

    Purpose

    TCR beta chain genomic transgenic construct for creating OT-1 mice

    Depositor

    Francis R. Carbone

    Article

    Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

    Insert

    TCR beta (Tcrb Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TR- ATG-HP-GFP

    Plasmid

    #80592

    Purpose

    Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Inserted 30 nt hairpin which is targeted by Csy4 …

    Promoter

    CBA

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TR-5'UTR-HP-GFP

    Plasmid

    #80594

    Purpose

    Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Inserted 30 nt hairpin which is targeted by Csy4 …

    Promoter

    CBA

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge