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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 144,362 results for: ins

Showing: 441 - 460 of 144362 results

  4. 36INS1-pBAD_AB

    Plasmid
    #78670
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78670-plasmid-map-sequence-id-147251

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  5. pJBL-RNAINS4

    Plasmid
    #69456
    Purpose
    RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S4 + SFGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    69456-plasmid-map-sequence-id-122578

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  6. pJBL-RNAINS3

    Plasmid
    #69455
    Purpose
    RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S3
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    mutations for S3 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    69455-plasmid-map-sequence-id-122579

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  7. Tg-UBC (TgTwinScan_2218)

    Plasmid
    #25565
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tg-UBC (TgTwinScan_2218)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. Escherichia coli DH10B-MOB

    Bacterial Strain
    #229395
    Purpose
    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)
    Depositor
    Michael Springer
    Article
    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1
    Bacterial Resistance
    60 ug/ml of dap
    Species
    Escherichia coli
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only

  9. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

    Plasmid
    #195048
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195048-plasmid-map-sequence-id-386137

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  10. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

    Plasmid
    #195047
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195047-plasmid-map-sequence-id-386131

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  11. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

    Plasmid
    #195046
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195046-plasmid-map-sequence-id-386136

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  14. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid
    #232344
    Purpose
    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag
    Depositor
    Philip Wong
    Article
    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10
    Insert
    HDGF Like 2 (HDGFL2 Human)
    Use
    Tags
    GGGS linker, 6xHis and Twin-strep, TEV protease s…
    Expression
    Mammalian
    Mutation
    TDP-43 regulated cryptic exon
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232344-plasmid-map-sequence-id-462328

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  18. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid
    #197264
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197264-plasmid-map-sequence-id-389660

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Showing: 441 - 460 of 144362 results