We narrowed to 2,317 results for: pET28

1. pET28B-PU.1-ETS-H3

   Plasmid

   #124435

   Purpose

   Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Residues 225 to 239 replaced with corresponding s…

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-INTS3-C

   Plasmid

   #128642

   Purpose

   Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   INTS3 C-termini (INTS3 Human)
   

   Use

   Tags

   His tag on N and C-terminal

   Expression

   Bacterial

   Mutation

   INTS3 C-termini (514–1042 aa)

   Promoter

   T7

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28B-PU.1-ETS-H3/S3

   Plasmid

   #124627

   Purpose

   Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 237 to 241 replaced with corresponding s…

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28B-PU.1-ETS-S3

   Plasmid

   #124550

   Purpose

   Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 242 to 246 replaced with corresponding s…

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28b-PU.1-ETS-H2

   Plasmid

   #123358

   Purpose

   Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Residues 207 to 215 replaced with corresponding s…

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-YJR129c

   Plasmid

   #85118

   Purpose

   bacterial expression of YJR129c

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFM3 (EFM3 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28B-Cwc25

   Plasmid

   #111340

   Purpose

   his tagged Cwc25

   Depositor

   Christine Guthrie

   Insert

   CWC25
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-HypD

   Plasmid

   #110288

   Purpose

   Expresses t-4-hydroxy-L-proline dehydratase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   t-4-Hydroxy-L-proline Dehydratase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-PDAE

    Plasmid

    #110180

    Purpose

    Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    Propanediol Dehydratase Activating Enzyme
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-mxEH

    Plasmid

    #104977

    Purpose

    Biosynthesis of oxylipins by microbial enzymes

    Depositor

    Deokkun Oh

    Insert

    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    

    Use

    Tags

    6 histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-PvuRts1I

    Plasmid

    #86888

    Purpose

    express C-term His tagged PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Use

    Tags

    C-term His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28b(+)-TM0848

    Plasmid

    #67287

    Purpose

    Expresses TM0848 in E. coli

    Depositor

    SeWon Suh

    Insert

    GTP-binding protein YqeH
    

    Use

    Tags

    N tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pET28b(+)-JHP933

    Plasmid

    #59302

    Depositor

    SeWon Suh

    Article

    Yoon et al Proteins. 2014 Mar 27. doi: 10.1002/prot

    Insert

    jhp0933
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    aa1-232

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET28b-LipB

    Plasmid

    #30165

    Depositor

    SeWon Suh

    Article

    Kim do et al Proteins. 2008 Mar . 70(4):1620-5.

    Insert

    lipoate protein ligase B
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-MED20

    Plasmid

    #15435

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED20 (Med20 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-MED11

    Plasmid

    #15414

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED11 (Med11 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pET28a(cd96)

    Plasmid

    #177901

    Purpose

    Bacterial expression of his10-tagged human cd96 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CD96 (aa 557-585 only) (CD96 Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28b-SpRY-His

    Plasmid

    #179320

    Purpose

    Plasmid for bacterial expression and purification of SpRY-Cas9

    Depositor

    Julián Cerón, Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28-MBP-TEV

    Plasmid

    #69929

    Purpose

    bacterial expression of MBP-TEV n-terminal fusions

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    MBP and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge