We narrowed to 1,799 results for: transfection

1. pSFV-Helper1

   Plasmid

   #92073

   Purpose

   This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

   Depositor

   Henrik Garoff

   Article

   Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

   Insert

   cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
   

   Use

   Helper plasmid, for run-off transcription and rna…

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Thy1.1-GFP

   Plasmid

   #131703

   Purpose

   encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

   Depositor

   Britt Glaunsinger

   Article

   Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

   Insert

   Thy1.1-GFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FosB

   Plasmid

   #187908

   Purpose

   Expresses FosB in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   FosB (Fosb Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. JunB

   Plasmid

   #187903

   Purpose

   Expresses JunB in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   JunB (Junb Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSFVCs-lacZ

   Plasmid

   #92076

   Purpose

   This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.

   Depositor

   Henrik Garoff

   Article

   Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112

   Insert

   C-lacZ
   

   Use

   C-lacz expression plasmid, for run-off transcript…

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. JunD

   Plasmid

   #187904

   Purpose

   Expresses JunD in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   JunD (Jund Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human USP30 (1-517, WT, MG-38-11)

   Plasmid

   #110748

   Purpose

   Transient transfection of human USP30

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC1812_pCR8-gCasRx-CUG (control gRNA)

   Plasmid

   #118645

   Purpose

   Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gCasRx-CUG
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-UbC-mCherry Rab5

   Plasmid

   #115239

   Purpose

   To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.

   Depositor

   Pantelis Tsoulfas

   Insert

   Rab5a (RAB5A Human, Homo sapiens)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Human UbC

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human USP30 (1-517, C77A, MG-38-16)

    Plasmid

    #110749

    Purpose

    Transient transfection of human USP30 C77A

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Expression

    Mammalian

    Mutation

    C77A

    Promoter

    CMV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Thy1.1-muSOX

    Plasmid

    #131702

    Purpose

    encodes Thy1.1 followed by a P2A site and muSOX for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-muSOX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmEGFP-N1 SYS1

    Plasmid

    #175092

    Purpose

    SYS1-mEGFP can be transfected in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Ishida et al J Cell Biol. 2019 Nov 4;218(11):3681-369

    Insert

    SYS1 (SYS1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(−) mCER-Prx2

    Plasmid

    #158224

    Purpose

    Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    mCER-Prx2
    

    Expression

    Mammalian

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC1819_pCR8-DR-SMN2-2-DR

    Plasmid

    #118653

    Purpose

    Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    DR-SMN2-2-DR
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC1820_pCR8-DR-RG6-SA-DR

    Plasmid

    #118654

    Purpose

    Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    DR-RG6-SA-DR
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. gRNA_CloningVector_tagBFP

    Plasmid

    #163747

    Purpose

    Empty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. gRNA_CloningVector_miRFP670

    Plasmid

    #163748

    Purpose

    Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid

    #208647

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV and U6 in tandem

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid

    #208979

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV and U6 in tandem

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSFV-Helper1

    Plasmid

    #92073

    Purpose

    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Insert

    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    

    Use

    Helper plasmid, for run-off transcription and rna…

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge