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pX330_SAFBgRNA9

Plasmid

#196108

Purpose

Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM1-RRM2-V5His6_H

Plasmid

#146490

Purpose

Insect Expression of DmPABPC1-RRM1-RRM2

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM1-RRM2 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM2-RRM3-V5His6_H

Plasmid

#146491

Purpose

Insect Expression of DmPABPC1-RRM2-RRM3

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM2-RRM3 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM3-RRM4-V5His6_H

Plasmid

#146492

Purpose

Insect Expression of DmPABPC1-RRM3-RRM4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM3-RRM4 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmPABPC1-RRM1-RRM2-V5His6_H

Plasmid

#146479

Purpose

Insect Expression of DmPABPC1-RRM1-RRM2

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM1-RRM2 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCav1 in pT3TS-Dest

Plasmid

#194293

Purpose

In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin (cav1.S Frog)

Use

In vitro transcription of mrna

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

Plasmid

#192233

Purpose

lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192234

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-2 lentiCRISPR v2 plasmid

Plasmid

#192232

Purpose

lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-1 lentiCRISPR v2 plasmid

Plasmid

#192231

Purpose

lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-DrCavin1a

Plasmid

#194291

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2F3-P229L-pET151

Plasmid

#177879

Purpose

Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Leucine (P229L)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2-P229S-pET151

Plasmid

#177871

Purpose

Expresses mouse Talin 1 F2-P229S domain in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2 P229S mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Serine (P229S)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2-P229L-pET151

Plasmid

#177876

Purpose

Expresses mouse Talin 1 F2-P229L domain in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2 P229L mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Leucine (P229L)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2F3-P229S-pET151

Plasmid

#177878

Purpose

Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Serine (P229S)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

Plasmid

#184636

Purpose

Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Mutation

Gat1 gene with Arginine 69 changed to Lysine, to …

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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3020_pETcon-SARS-CoV-2-RBD_N501Y

Plasmid

#184405

Purpose

yeast surface display of the SARS-CoV-2 Alpha variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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ETS2_pLENTI-CAG-IRES-GFP

Plasmid

#177005

Purpose

Mammalian lentiviral expression vector encoding ETS2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ETS2 (ETS2 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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MOSMO_pcDNA6.2/EmGFP-Bsd

Plasmid

#176975

Purpose

Mammalian expression vector encoding MOSMO and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MOSMO (C16orf52 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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