We narrowed to 23,734 results for: promoter

1. pJH131

   Plasmid

   #48260

   Purpose

   Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

   Inserts

   URA3 3' fragment
   URA3 5' fragment
   GAL10/GAL1 bidirectional promoter
   

   Use

   Routine cloning vector

   Mutation

   -242 upstream to URA3 ORF +495 and URA3 ORF from …

   Promoter

   GAL10/GAL1 bidirectional

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF6 rglut1 HA complete

   Plasmid

   #89572

   Purpose

   rglut1 HA complete

   Depositor

   Jeffrey Rathmell

   Article

   Wieman et al Mol Biol Cell. 2007 Apr;18(4):1437-46. E

   Insert

   pEF6 rglut1 HA complete (Slc2a1 Rat)
   

   Tags

   V5-His A, B, C and rglut1 HA complete

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFIN-IRBP1783-CHER

   Plasmid

   #44357

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Insert

   IRBP1783-CHER
   

   Use

   Lentiviral

   Promoter

   murine interphotoreceptor retinoid-binding protei…

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBR322-bom

   Plasmid

   #155178

   Purpose

   pBICI_2_strong without bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE9-His-p97deltaD2

   Plasmid

   #17229

   Depositor

   Hemmo Meyer

   Article

   Ramadan et al Nature. 2007 Dec 20. 450(7173):1258-62.

   Insert

   p97 (Vcp Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed codon 457 to stop codon

   Available Since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pBP-Bba_K822000

   Plasmid

   #74094

   Purpose

   L-Lactate inducible promoter from E. coli MG1655

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Bba_K822000 (L-Lactate promoter - E. coli MG1655)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   NA

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEBB-3XMyc-TRAF2 S11A

   Plasmid

   #44105

   Depositor

   William Hahn

   Article

   Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

   Insert

   TRAF2 S11A (TRAF2 Human)
   

   Tags

   3xMyc

   Expression

   Mammalian

   Mutation

   Serine 11 to Alanine

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pRK-flag-GFP 1-10 tetra Cys

   Plasmid

   #78589

   Purpose

   Expression flag-GFP 1-10 tetra Cys in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-GFP 1-10 tetra Cys
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCK300

   Plasmid

   #87766

   Purpose

   empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   None

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM6-G6-mCherry

    Plasmid

    #66533

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter G6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - G6

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid

    #164266

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6 promoter from W05B2.8

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. attB-P7-attP-YFP

    Plasmid

    #195571

    Purpose

    A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.

    Depositor

    Richard Murray

    Article

    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

    Insert

    Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
    

    Use

    Synthetic Biology

    Promoter

    P7

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. secCitrine

    Plasmid

    #181964

    Purpose

    Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    signal peptide sequence from basic chitinase
    

    Tags

    Citrine YFP

    Expression

    Plant

    Promoter

    1.5 kb MUM4 promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3_4xUAS_CP-candidate_luc+

    Plasmid

    #125154

    Purpose

    STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Luciferase

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KG#707

    Plasmid

    #110911

    Purpose

    Expresses the C. elegans sup-1(e995) minigene in ventral nerve cord cholinergic motor neurons

    Depositor

    Kenneth Miller

    Article

    Harterink et al J Cell Sci. 2018 Oct 22;131(20). pii: jc

    Insert

    sup-1 minigene
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXD70LacZ3-Pcsd-LacZ

    Plasmid

    #191619

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoter

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFlox_N-BRG1_Neo_FKBPF36V_mScarlet

    Plasmid

    #163875

    Purpose

    Acute Depletion of BRG1-mScarlet

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    Brg1 (Smarca4 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-H9-mCherry

    Plasmid

    #66532

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H9

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H9

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcry:GFP,-503unc:hACTA1GFP

    Plasmid

    #172529

    Purpose

    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.

    Depositor

    Joachim Berger

    Article

    Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

    Insert

    ATCA1
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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