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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,065 results for: LEA

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Showing: 4601 - 4620 of 14065 results

  1. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid
    #223113
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223113-plasmid-map-sequence-id-447636

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  2. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

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  17. pST50Trc3-STRNDHFR

    Plasmid
    #63978
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63978-plasmid-map-sequence-id-177384

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  18. JDS80

    Plasmid
    #40316
    Depositor
    Keith Joung
    Type
    Empty backbone
    Tags
    3XFLAG and FOK1 KKR (+) heterodimer
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 16, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40316-plasmid-map-sequence-id-51984

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  19. JDS90

    Plasmid
    #40320
    Depositor
    Keith Joung
    Type
    Empty backbone
    Tags
    3XFLAG and FOK1 ELD (-) heterodimer
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 26, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40320-plasmid-map-sequence-id-51988

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  20. ACV

    Plasmid
    #27791
    Depositor
    Steven Vogel
    Publication
    Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amber-Cerulean-Venus
    Use
    Stoichiometry standard
    Expression
    Mammalian
    Available since
    June 23, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 4601 - 4620 of 14065 results