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pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

Plasmid

#139086

Purpose

Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp16

Plasmid

#157724

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp16 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TRAV16

Plasmid

#123389

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV16 (TRAV16 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV13-1

Plasmid

#123386

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV13-1 (TRAV13-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

Plasmid

#194692

Purpose

hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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aMHC-Puro Rex-Blast

Plasmid

#21231

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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myc-KIF5A

Plasmid

#127616

Purpose

N-terminal myc-tagged mouse KIF5A

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5A (Kif5a Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast-CDT1-HA

Plasmid

#109335

Purpose

virus packaged, doxycyline inducible expression of Cdt1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cdt1 (CDT1 Human)

Use

Lentiviral; Inducible expression

Tags

HA, His, Strep

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CCR5

Plasmid

#98948

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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VZV ORF 60 (gL)

Plasmid

#64082

Purpose

eukaryotic expression plasmid for VZV glycoprotein L

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein L

Expression

Mammalian

Promoter

CMV IE with intron

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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TRBV18

Plasmid

#123452

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV18 (TRBV18 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV15

Plasmid

#123450

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV15 (TRBV15 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV10-3

Plasmid

#123441

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV10-3 (TRBV10-3 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV7-2

Plasmid

#123431

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV7-2 (TRBV7-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV6-1

Plasmid

#123421

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV6-1 (TRBV6-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV4-2

Plasmid

#123412

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV4-2 (TRBV4-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV4-1

Plasmid

#123411

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV4-1 (TRBV4-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV38-2

Plasmid

#123405

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV38-2 (TRAV38-2DV8 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV22

Plasmid

#123394

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV22 (TRAV22 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV20

Plasmid

#123392

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV20 (TRAV20 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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