We narrowed to 15,034 results for: invs

1. pVITRO1-TagRFP-NES

   Plasmid

   #184449

   Purpose

   Cytosolic TagRFP expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   TagRFP-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-mScarlet-NS

   Plasmid

   #140874

   Purpose

   Multisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   mScarlet
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Aahn

   Plasmid

   #112158

   Purpose

   A genetically encoded voltage indicator (Aahn), capable of optically monitoring both plasma membrane and internal membrane potentials.

   Depositor

   Bradley Baker

   Article

   Sepehri Rad et al Sci Rep. 2018 May 2;8(1):6911. doi: 10.1

   Insert

   Aahn
   

   Use

   Tags

   Super ecliptic pHlorin A227D

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-L3-oPRE-L2

   Plasmid

   #32415

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   oPRE
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   WPRE with deletion of X protein promoter and muta…

   Promoter

   Available Since

   Jan. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. cacna2d1 R241A D491A HA pcDNA3

   Plasmid

   #206109

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding

   Depositor

   Annette Dolphin

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R241A D491A

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Wnt3-V5

   Plasmid

   #35928

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt3 (WNT3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 10 mut B-Myb

   Plasmid

   #25966

   Depositor

   Rob Lewis

   Article

   Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

   Insert

   B-Myb (Mybl2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed threonines 267, 408, 497, 519, 522 and 52…

   Promoter

   Available Since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pSABAD505

   Plasmid

   #45615

   Depositor

   Hideo Iwai

   Article

   Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

   Insert

   GFPN-NpuDnaE_intein-GFP_C
   

   Use

   Tags

   GFP_C and GFP_N

   Expression

   Bacterial

   Mutation

   stemmer GFP with superfolder mutations S30R and Y…

   Promoter

   pBAD

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Wnt11 STOP

   Plasmid

   #35885

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   WNT11 (WNT11 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   None

   Promoter

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-calx1

    Plasmid

    #58697

    Purpose

    Expression of calx1

    Depositor

    Craig Montell

    Article

    Wang et al Neuron 2005 Feb 3;45(3):367-78.

    Insert

    calx1 (Calx Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

11. nonphosphorylatable Mis12-targeted Aurora B FRET sensor

    Plasmid

    #45229

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    non phosphorylatable Mis12-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T-A mutation in substrate sequence

    Promoter

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRL8

    Plasmid

    #24980

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

    Insert

    Cbr-unc-119
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SMAD2_HAe2_Bsd

    Plasmid

    #167166

    Purpose

    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.

    Depositor

    Alexander Loewer

    Insert

    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    

    Use

    Repair template for gene editing in human cells

    Tags

    blasticidin

    Expression

    Mutation

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CENP-B mCherry PP1 gamma

    Plasmid

    #45221

    Depositor

    Iain Cheeseman, Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    CENP-B mCherry PP1 gamma
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Kohinoor-Paxillin/pcDNA3

    Plasmid

    #67775

    Purpose

    Paxilin-targetted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor
    

    Use

    Tags

    Paxillin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-Pol-II 15

    Plasmid

    #35177

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains the C-terminal repeats 16-52

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3 AU1 mTOR S2035W

    Plasmid

    #15678

    Depositor

    John Lawrence

    Article

    McMahon et al Mol Cell Biol. 2002 Nov . 22(21):7428-38

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    S2035W

    Promoter

    Available Since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pLO104

    Plasmid

    #90224

    Purpose

    Complements mutations in Aspergillus nidulans gene riboB (AN0670) (mutants require riboflavin)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_00531
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid

    #69548

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR/pTER shLUC (w177-1)

    Plasmid

    #17472

    Purpose

    Gateway entry vector for Firefly Luciferase shRNA under H1/TO promoter

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase shRNA
    

    Use

    RNAi; Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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