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pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

Plasmid

#61582

Purpose

Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne2 (OTUD7A Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Isoform 2. Deleted aa 463-933.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-STOP

Plasmid

#140876

Purpose

Multisite Gateway middle entry vector for expression of untagged mScarlet.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway Cloning

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-CaaX(tH)

Plasmid

#109539

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Hras

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75177

Purpose

Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-tdTomato-FlagAkt2W80A

Plasmid

#64834

Purpose

cDNA of Akt2 with point mutation from Trp to Ala at position 80. This mutation confers resistance to MK-2206 drug.

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt2 (Akt2 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Point mutation Trp 80 to Ala

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
mVenusC-Cavin1

Plasmid

#108888

Purpose

Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin1 (altered codon usage) (CAVIN1 Human, Synthetic)

Tags

mVenus156-239

Expression

Mammalian

Mutation

altered codon usage

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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mVenusN-Cavin1

Plasmid

#108887

Purpose

Expression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

caveolae associated protein 1 (CAVIN1 Human, Synthetic)

Tags

mVenus1-155

Expression

Mammalian

Mutation

altered codon usage

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTalpha1-EGFP-N1

Plasmid

#133929

Purpose

Neuron-specific expression of EGFP

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP

Expression

Mammalian

Mutation

EGFP

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDC

Plasmid

#166941

Purpose

Dual control based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Circuit expressing GFP- Dual Control

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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MIS12-FRB-FLAG

Plasmid

#114040

Purpose

Kinetochore localised FRB tagged with FLAG

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

MIS12 (MIS12 Human)

Tags

FRB-FLAG-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pME-mVenus-NS

Plasmid

#80497

Purpose

Multisite gateway entry clone for adding N-terminal fusions of mVenus

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

mVenus (no STOP)

Use

Multisite gateway middle entry clone

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dSesn (1187)

Plasmid

#66919

Purpose

bacterial expression of dSesn

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn (Sesn Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP2-NS

Plasmid

#80502

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP2

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

TagBFP2 (no STOP)

Use

Multisite gateway middle entry clone

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTKDP-hph

Plasmid

#71322

Purpose

Donor plasmid - hygromycin resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pVB002

Plasmid

#127186

Purpose

Tunable NOT gate with RiboJ insulator

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate with RiboJ and Reporter

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVB005

Plasmid

#127189

Purpose

Tuner sRNA booster

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

sRNA Booster

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-csGBP

Plasmid

#108891

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

csGBP

Use

Gateway Cloning

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75154

Purpose

Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Biomolecular FeedBack (FB)

Plasmid

#109391

Purpose

This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pJ23100mut-dCas9-T- phtpG1-sgRNA

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcSrc416/527

Plasmid

#17677

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y416F Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Tyr 416 changed to Phe . Tyr 527 changed to Phe .

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only