We narrowed to 11,588 results for: VARS

1. Lenti-Nova1-GFP

   Plasmid

   #217033

   Purpose

   Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

   Depositor

   Chaolin Zhang

   Article

   Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

   Insert

   Nova1 (Nova1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLINE1ΔORF2-scFvFc

   Plasmid

   #206021

   Purpose

   The ORF2 sequence was removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
   

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLINE1ΔORF1-2-scFvFc

   Plasmid

   #206022

   Purpose

   The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-CMV-jGCaMP8f L27A

   Plasmid

   #204140

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f L27A
   

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Leucine 27 changed to Alanine

   Promoter

   CMV

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-DDK_V222A RAD23B

   Plasmid

   #201580

   Purpose

   Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation V222A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6-AN-DDK_F400A RAD23B

   Plasmid

   #201581

   Purpose

   Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation F400A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28 sf:em-blue-shift.1

   Plasmid

   #191928

   Purpose

   Encodes a GFP design, sf:em-blue-shift.1

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:em-blue-shift.1
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S Q69P S72A T108V Y145M T167V V224I

   Promoter

   T7

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL0_AC-27877

   Plasmid

   #202110

   Purpose

   Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   27877 promoter
   

   Use

   Synthetic Biology

   Mutation

   Promoter sequence mutated to remove sapI sites fo…

   Available Since

   Aug. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pL0_CE-OriT

   Plasmid

   #202150

   Purpose

   Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Origin of transfer
   

   Use

   Synthetic Biology

   Mutation

   OriT sequence mutated to remove sapI sites domest…

   Available Since

   Aug. 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pL0_AC-Fld

    Plasmid

    #202125

    Purpose

    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Fld promoter
    

    Use

    Synthetic Biology

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Available Since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pL0_AC-54730

    Plasmid

    #202109

    Purpose

    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    54730 promoter
    

    Use

    Synthetic Biology

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pL0_AC-51183

    Plasmid

    #202108

    Purpose

    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    51183 promoter
    

    Use

    Synthetic Biology

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pL0_AC-GEF

    Plasmid

    #202107

    Purpose

    Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    GEF promoter
    

    Use

    Synthetic Biology

    Mutation

    Promoter sequence mutated to remove sapI site for…

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pL0_AC-47740

    Plasmid

    #202106

    Purpose

    Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    47740 promoter
    

    Use

    Synthetic Biology

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pL0_AC-BCA

    Plasmid

    #202105

    Purpose

    Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    BCA promoter
    

    Use

    Synthetic Biology

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pL0_AC-epyC

    Plasmid

    #202104

    Purpose

    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    epyC promoter
    

    Use

    Synthetic Biology

    Mutation

    Promoter sequence mutated to remove bsaI sites fo…

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

    Plasmid

    #197426

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ230-D156R

    Plasmid

    #188542

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible lbcas12a-d156r entry c…

    Tags

    NLS

    Expression

    Plant

    Mutation

    D156R

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sniper2P

    Plasmid

    #193857

    Purpose

    Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

    Insert

    Sniper2L
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    E1007L

    Promoter

    EFS

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CABE-T1.14

    Plasmid

    #193259

    Purpose

    Expression of CABE-T1.14 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T1.14
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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