We narrowed to 8,355 results for: construct

1. CSK22_HUMAN_D0

   Plasmid

   #79704

   Purpose

   This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSK22 (CSNK2A2 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. KIlac

   Bacterial Strain

   #52696

   Purpose

   MG1655 with Lac Operon removed from native locus and re-integrated at the attTn7 locus

   Depositor

   Tanja Kortemme

   Article

   Eames et al Science. 2012 May 18;336(6083):911-5. do

   Bacterial Resistance

   None

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

3. C321.ΔClpS.Ub-UAG-sfGFP

   Bacterial Strain

   #98565

   Purpose

   Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivated

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Bacterial Resistance

   Ampicillin

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pDUAL SRBI (GFP)

   Plasmid

   #86979

   Purpose

   Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   SCARB1 (SCARB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV/hPGK

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cre Shine

   Plasmid

   #37404

   Purpose

   This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.

   Depositor

   Thomas Hughes

   Insert

   Cre Shine
   

   Use

   Cre/Lox

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-fDIO-mCherry-WPREpA

   Plasmid

   #177328

   Purpose

   Control vector for Flp-dependent DREADD constructs

   Depositor

   Ulrik Gether

   Insert

   mCherry
   

   Use

   AAV; Flp-frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin-1

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBHM2642

   Plasmid

   #229526

   Purpose

   Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   Tags

   t2a-mNeonGreen-mIAA7_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. FU_tetO_dCas9-VP192-T2A-EGFP

   Plasmid

   #121173

   Purpose

   Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

   Depositor

   Timo Otonkoski

   Insert

   dCas9-VP192-T2A-EGFP
   

   Use

   CRISPR and Lentiviral; Inducible by doxycycline

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hsp68-LacZ-Gibson

   Plasmid

   #170102

   Purpose

   Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.

   Depositor

   Len Pennacchio

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Mouse Hsp68 minimal promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. WARS_pNIC-CTB10H

    Plasmid

    #153058

    Purpose

    Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    WARS (WARS1 Human)
    

    Use

    Tags

    TEV-Avi-His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. katushka-FMDV-P2A-pTRIP

    Plasmid

    #163522

    Purpose

    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

    Depositor

    Thomas Weber

    Insert

    stopless Katushka-P2A
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKT-PDGFRa-IRES-Katushka

    Plasmid

    #133081

    Purpose

    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    PDGFRa (Pdgfra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. YARS_pNIC-CTB10H

    Plasmid

    #153059

    Purpose

    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    YARS (YARS1 Human)
    

    Use

    Tags

    TEV-Avi-His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid

    #172296

    Purpose

    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

    Depositor

    Patrik Verstreken

    Insert

    Cas9n-RT-T2A-PuroR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    H840A (Cas9)

    Promoter

    T7 promoter A-initiating

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPK719

    Plasmid

    #38149

    Depositor

    Patricia Kuwabara

    Insert

    cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)
    

    Use

    Recombineering

    Tags

    Expression

    Worm

    Mutation

    Promoter

    unc-119

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTRY4_ Pi21

    Plasmid

    #126904

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBHM2643

    Plasmid

    #229527

    Purpose

    Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mAID_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid

    #221536

    Purpose

    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. L0-T7 Circuit 2 GG1-PhiC31P-bbs1-BxB1P-GG2

    Plasmid

    #219692

    Purpose

    Level 0 to construct history-dependent target T2BP

    Depositor

    Jennifer Nemhauser

    Insert

    GG1-PhiC31P-bbs1-BxB1P-GG2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2

    Plasmid

    #219691

    Purpose

    Level 0 to construct history-dependent target T2PB

    Depositor

    Jennifer Nemhauser

    Insert

    GG1-Bxb1P-bbs1-PhiC31P-GG2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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