We narrowed to 26,486 results for: vit;

1. pLKO.1P DNA2_1

   Plasmid

   #160779

   Purpose

   Suppress DNA2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shDNA2_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1P BRIP1_2

   Plasmid

   #160772

   Purpose

   Suppress BRIP1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shBRIP1_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1P BRIP1_1

   Plasmid

   #160771

   Purpose

   Suppress BRIP1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shBRIP1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1B CDC25C_2

   Plasmid

   #160770

   Purpose

   Suppress CDC25C

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCDC25C_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1B CDC25C_1

   Plasmid

   #160769

   Purpose

   Suppress CDC25C

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCDC25C_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1B CDC25A_1

   Plasmid

   #160767

   Purpose

   Suppress CDC25A

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCDC25A_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT140_GalL_cCDA1-VRERBE3

   Plasmid

   #145103

   Purpose

   Expressing base editor cCDA1-VRERBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-VRERBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

   Promoter

   GalL

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

   Plasmid

   #123354

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   cp-moxCerulean3-moxCerulean3-FLARE-D1ER
   

   Tags

   C-terminal ER retention signal (KDEL), cp-moxCeru…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT176_GalL_eA3A-BE3

   Plasmid

   #145125

   Purpose

   Expressing base editor eA3A-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(N57G)-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   A3A(N57G); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT174_GalL_A3A-Y130F-BE3

    Plasmid

    #145123

    Purpose

    Expressing base editor A3A-Y130F-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(Y130F)-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(Y130F); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT173_GalL_A3A-R128A-BE3

    Plasmid

    #145122

    Purpose

    Expressing base editor A3A-R128A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(R128A)-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(R128A); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT122_GalL_cCDA1-VQRBE3

    Plasmid

    #145087

    Purpose

    Expressing base editor cCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VQRBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT113_GalL_cCDA1-xBE3

    Plasmid

    #145079

    Purpose

    Expressing base editor cCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-xBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126758

    Purpose

    Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126759

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126753

    Purpose

    Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126754

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126756

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAWp63-clone3-12 (OptiHF-SpCas9)

    Plasmid

    #131737

    Purpose

    Expresses OptiHF-SpCas9 in mammalian cells

    Depositor

    Alan Siu Lun Wong

    Article

    Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

    Insert

    OptiHF-SpCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    Q695A+K848A+E923M+T924V+Q926A

    Promoter

    EFS

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Met133I, M1381I, M186I ELL2

    Plasmid

    #127263

    Purpose

    For in vitro translation of human ELL2 with Met133I, M1381I, M186I

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Three Mets (133, 138, 186) to Ileu

    Promoter

    T7

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONRG_P4-P1R:TBA2p

    Plasmid

    #128429

    Purpose

    Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Insert

    TESTA ABUNDANT 2 (AT1G62060 Mustard Weed)
    

    Use

    Gateway promoter entry clone

    Promoter

    TESTA ABUNDANT 2

    Available Since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. Designed HEL-binding scFv D44.1des

    Plasmid

    #111719

    Purpose

    Yeast Surface Display of D44.1des

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    D44.1 des
    

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HA tagged Sph ELL2

    Plasmid

    #127270

    Purpose

    For in vitro translation of human WT ELL2 with internal HA tag

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    WT Mets and HA tag at SphI site (8th Met)

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Met138ILeu ELL2

    Plasmid

    #127260

    Purpose

    For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met138 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Met186ILeu ELL2

    Plasmid

    #127261

    Purpose

    For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met186 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met186 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Met138ILeu, Met186ILeu ELL2

    Plasmid

    #127265

    Purpose

    For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Two Mets (138,186) to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Met133ILeu ELL2

    Plasmid

    #127259

    Purpose

    For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met133 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Met133ILeu, Met186ILeu ELL2

    Plasmid

    #127264

    Purpose

    For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Two Mets (133,186 )to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Met331Leu ELL2

    Plasmid

    #127262

    Purpose

    For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met331 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met331 to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Met1ILeu ELL2

    Plasmid

    #127258

    Purpose

    For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met1 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met1 to Ileu by Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ELL2mRNA+polyA

    Plasmid

    #127256

    Purpose

    For in vitro translation of human ELL2 with 30 nt pA tail

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    shortens 3'UTR to 40 bp adds A30 tail

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HA tagged Xba ELL2

    Plasmid

    #127268

    Purpose

    For in vitro translation of WT ELL2 with HA tag inserted near N-term

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

    Promoter

    T7

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

33. pMCh1809_pBS_cdc42E6-boxB

    Plasmid

    #118609

    Purpose

    cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E6 3'UTR (Cdc42 Mouse)
    

    Use

    In vitro transcription

    Tags

    boxB

    Promoter

    T3

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMCh1807_pBS_cdc42E7-boxB

    Plasmid

    #118612

    Purpose

    cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7 3'UTR (Cdc42 Mouse)
    

    Use

    In vitro transcription

    Tags

    boxB

    Promoter

    T3

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHTSHP-ShhN-Cys

    Plasmid

    #121136

    Purpose

    Expresses ShhN with a C-terminal cysteine residues in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    shh (Shh Mouse)
    

    Tags

    HIS, HRV3C site, and MBP

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-191, with an addition…

    Promoter

    tac

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMCh-1498

    Plasmid

    #82427

    Purpose

    Plasmid for expression of NHA-tagged Nematostella vectensis GW182 11W11A in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 11W11A
    

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    Trp 1167, 1183, 1227, 1309, 1391, 1453, 1574, 158…

    Promoter

    CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMCh-1539

    Plasmid

    #82432

    Purpose

    Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    hs-nvGW182 chimera
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

    Promoter

    EF1A

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMCh-1537

    Plasmid

    #82430

    Purpose

    Plasmid for expression of NHA-tagged human-Nematostella GW182 chimera in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    hs-nvGW182 chimera
    

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

    Promoter

    CMV

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. non-targeting control gRNA (BRDN0001146109)

    Plasmid

    #80242

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. non-targeting control gRNA (BRDN0001146001)

    Plasmid

    #80243

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge