We narrowed to 15,877 results for: ADA

1. pcDNA5FRT/TO-Npl4-Strep-HA

   Plasmid

   #113495

   Purpose

   Expression of human Npl4 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Npl4 (NPLOC4 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-TRC.mKO2_shGFP

   Plasmid

   #110318

   Purpose

   Control (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXJ42-p200 CUX1

   Plasmid

   #100813

   Purpose

   Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

   Depositor

   Alain Nepveu

   Article

   Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. do

   Insert

   p200 CUX1 (CUX1 Human)
   

   Use

   Note that this plasmid does not have an sv40 ori

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

   Plasmid

   #90332

   Purpose

   CRISPR interference

   Depositor

   Dirk Heckl

   Article

   Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

   Inserts

   dCas9
   na
   SFFV
   P2A-BSD
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT/TO-Ufd1-Strep-HA

   Plasmid

   #113474

   Purpose

   Expression of human Ufd1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Ufd1 (UFD1 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSH-EFIRES-P-AtAFB2-mCherry-weak NLS

   Plasmid

   #129717

   Purpose

   AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry-weak NLS

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPR v2-sgACSL4 clone2

   Plasmid

   #162129

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPR v2-sgACSL4 clone1

   Plasmid

   #162128

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-cFos-XRI-V5

   Plasmid

   #178058

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-V5
   

   Use

   AAV

   Tags

   V5-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse c-fos promoter

   Available since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-Blast HA-POLE2

    Plasmid

    #160809

    Purpose

    Express HA-tagged POLE2

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    HA-POLE2 (POLE2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

    Plasmid

    #178528

    Purpose

    Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    rtTA, SspB-mScarlet-I-MYPT (1-169aa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, Tet responsive element

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC_hfnCas12a_CRATES

    Plasmid

    #176251

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Integrin beta5-2XEGFP

    Plasmid

    #139779

    Purpose

    Detecting Integrin beta5 by fluorescence microscopy

    Depositor

    Staffan Stromblad

    Article

    Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

    Insert

    Integrin subunit beta5 (ITGB5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-EGFP-AID-BubR1

    Plasmid

    #47330

    Purpose

    for plasmid integration using the Flp-In system. AID at N-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    AID and GFP

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Promoter

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI-neo-RL-ZEB2 200bmut

    Plasmid

    #35538

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB2 gene (ZEB2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    6 Mutations in the miR-200b seed regions of the Z…

    Promoter

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVH-EF1a-GFP-P2A-KLF4

    Plasmid

    #130694

    Purpose

    Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

    Plasmid

    #171150

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-P2A-H2B-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

    Plasmid

    #176250

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

    Plasmid

    #101064

    Purpose

    AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2_L398T
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-SaPE2

    Plasmid

    #174817

    Purpose

    Mammalian expression of SaCas9 prime editor 2

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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