We narrowed to 10,352 results for: FIE

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pDONR223_GPS2_WT

Plasmid

#81816

Purpose

Gateway Donor vector containing GPS2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GPS2 (GPS2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_H2AFX_WT

Plasmid

#81937

Purpose

Gateway Donor vector containing H2AFX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

H2AFX (H2AX Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EZH2_WT

Plasmid

#81742

Purpose

Gateway Donor vector containing EZH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EZH2 (EZH2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only
pDONR223_STAT3_p.Y705F

Plasmid

#82086

Purpose

Gateway Donor vector containing STAT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway Cloning

Mutation

Y705F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AXIN2_WT

Plasmid

#82099

Purpose

Gateway Donor vector containing AXIN2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AXIN2 (AXIN2 Human)

Use

Gateway Cloning

Mutation

P50S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBFC0996

Plasmid

#186695

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
crRNA
Tn7 transposon
2xLguI Cargo Stuffer
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFB2

Plasmid

#195282

Purpose

Bicistronic DTR and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR 2A Puro (HBEGF Synthetic, Human)

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETS1_WT

Plasmid

#82118

Purpose

Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ETS1 (ETS1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

Plasmid

#160628

Purpose

Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos, 35S, 35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_FYN_WT

Plasmid

#82211

Purpose

Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FYN (FYN Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NOTCH1_ICN

Plasmid

#82087

Purpose

Gateway Donor vector containing NOTCH1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NOTCH1 (NOTCH1 Human)

Use

Gateway Cloning

Mutation

ICN (AA1761-2556 present)

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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COVID-SARS2 NSP14/10

Plasmid

#159613

Purpose

Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

Depositor

Opher Gileadi

Article

Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

Inserts

Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
Covid-SARS2 Nsp10

Tags

His6, TEV cleavage site and none

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

(Bicistronic) and T7 - LacO

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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sgLATS1/2-1

Plasmid

#229431

Purpose

knockout of LATS1 and LATS2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgLATS1/2-1 (LATS1, LATS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT1_WT

Plasmid

#82234

Purpose

Gateway Donor vector containing STAT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT1 (STAT1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CD70_WT

Plasmid

#82003

Purpose

Gateway Donor vector containing CD70, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CD70 (CD70 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDKN1A_WT

Plasmid

#82201

Purpose

Gateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1A (CDKN1A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIP-mCherry-Halo-YAP1

Plasmid

#128336

Purpose

Retrovirus construct to express mCherry-Halo-YAP1

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Insert

YAP1 (YAP1 Human)

Use

Retroviral

Tags

mCherry and Halo-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Sox2 HM a

Plasmid

#26353

Depositor

Matthew Meyerson

Article

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

Sox2 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 IGF1R-BirA(R118G)-HA

Plasmid

#109232

Purpose

This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

Depositor

Victor Dzau

Article

Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

Insert

IGF1R (IGF1R Human)

Tags

BirA(R118G) (highly promiscuous form) and HA

Expression

Mammalian

Promoter

T7

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_SETDB1_WT

Plasmid

#82291

Purpose

Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SETDB1 (SETDB1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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