We narrowed to 10,674 results for: cat.1

1. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

   Plasmid

   #115698

   Purpose

   ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

   Depositor

   Eckhard Boles

   Article

   Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

   Insert

   SSKar2-sfpHluorin-HDEL
   

   Use

   Tags

   ER retention signal containing HDEL and ER signal…

   Expression

   Yeast

   Mutation

   for introduced mutations see publication

   Promoter

   MET25

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pegRNA-GBA-10

   Plasmid

   #199271

   Purpose

   pegRNA used to induce GBA (c. 1226 A > G) mutation

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   GBA 1226AtoG pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GreenT-EC - GPI

   Plasmid

   #193946

   Purpose

   Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC
   

   Use

   Tags

   GPI-anchor and Igk_secretion - HA-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

   Plasmid

   #168192

   Purpose

   HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-LaG6-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MLSE-shREN

   Plasmid

   #105583

   Purpose

   retrovirally express control shRNA with GFP marker

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   control shRNA targeting luciferase
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTR

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-crRNA-Km

   Plasmid

   #158712

   Purpose

   Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   AcGFP (for crRNA cloning)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEE393

   Plasmid

   #149283

   Purpose

   T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

8. rat APOBEC1

   Plasmid

   #112858

   Purpose

   plasmid for expression of rat APOBEC1 in mammalian cells

   Depositor

   Silvestro Conticello

   Article

   Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-MTS-FLAG-LOV*

   Plasmid

   #202207

   Purpose

   Expresses the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

   Depositor

   Thomas Muir

   Article

   Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
   

   Use

   Tags

   FLAG and LOV*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-CAG-Nanog-pA-pgk-hph

    Plasmid

    #74915

    Purpose

    Mammalian expression of mNanog

    Depositor

    Jose Silva

    Article

    Theunissen et al Curr Biol. 2011 Jan 11;21(1):65-71. doi:

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pEE389

    Plasmid

    #149287

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

13. pZA13

    Plasmid

    #158491

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    no STOP codon

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_TEAD3_1

    Plasmid

    #86337

    Purpose

    Encodes gRNA for 3' target of human TEAD3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against TEAD3 (TEAD3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEE385

    Plasmid

    #149289

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUPER-ARF5b

    Plasmid

    #67500

    Purpose

    Expression of shRNA targeting human ARF5b

    Depositor

    Richard Kahn

    Article

    Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

    Insert

    HsARF5 shRNA (ARF5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. gRNA[bTub].1026C

    Plasmid

    #112691

    Purpose

    express gRNA targeting bTub under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    dU6-3

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid

    #202588

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, changed O…

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GreenT-EC - pRSET

    Plasmid

    #193947

    Purpose

    Bacterial expression vector for a low-affinity calcium indicator

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GreenT-EC.b - pRSET

    Plasmid

    #193948

    Purpose

    Bacterial expression vector for a low-affinity calcium indicator

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.b
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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