We narrowed to 20,413 results for: pcDNA

1. pcDNA3-Myc-IQGAP1

   Plasmid

   #30118

   Depositor

   David Sacks

   Article

   Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

   Insert

   IQGAP1 (IQGAP1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-Rho*-BERKY3

   Plasmid

   #158220

   Purpose

   BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-Gα13*-BERKY3

   Plasmid

   #158218

   Purpose

   BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Flag-Tet1 CD

   Plasmid

   #70129

   Purpose

   Express flag-tagged mouse Tet1 catalytic domain.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET1 catalytic domain.

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-IGF1R-V5/HIS

   Plasmid

   #201979

   Purpose

   expression of human IGF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   IGF1R receptor tyrosine kinase (IGF1R Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-hPTGS2-2flag

   Plasmid

   #102498

   Purpose

   Mammalian expression of PTGS2

   Depositor

   Jun Yu

   Article

   Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

   Insert

   PTGS2 (PTGS2 Human)
   

   Use

   Tags

   Flag-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-N-Flag-Caspase-1

   Plasmid

   #75128

   Purpose

   Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   caspase 1 (Casp1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   P341S in Caspase-1 (please see depositor comments…

   Promoter

   CMV

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. N370S GBA pcDNA3.1

   Plasmid

   #188583

   Purpose

   Ectopic expression N370S GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N370S

   Promoter

   CMV

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1/CALR wt-FLAG

   Plasmid

   #204532

   Purpose

   Mammalian expression of human CALR wt-FLAG

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   CALR wt-FLAG (CALR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-Hygro(+)-mscGAS

    Plasmid

    #102607

    Purpose

    Mammalian expression of mouse cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (Cgas Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L444P GBA pcDNA3.1

    Plasmid

    #188582

    Purpose

    Ectopic expression L444P GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L444P

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA4-FLAG-Jhdm2a

    Plasmid

    #38136

    Depositor

    Toru Nakano, Toshinobu Nakamura

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-DN-hCUL1-FLAG

    Plasmid

    #15818

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin1 (CUL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N452

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 Neo(+) hSUMF1

    Plasmid

    #24386

    Depositor

    Carolyn Bertozzi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

    Insert

    hSUMF1 (SUMF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1.Gαi1-LgB91

    Plasmid

    #134342

    Purpose

    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi1-LgB91 (GNAI1 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. WFS1-Flag-pcDNA3

    Plasmid

    #13011

    Depositor

    Fumihiko Urano

    Article

    Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

    Insert

    Wolfram Syndrome 1 (WFS1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-EGFP-Rac1(wt)

    Plasmid

    #13719

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    EGFP-Rac1(wt) (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-CRISPR-Puro

    Plasmid

    #208646

    Purpose

    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-GGamma2-GFP2

    Plasmid

    #166774

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma2-GFP2 (GNG2 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge