We narrowed to 8,355 results for: construct

1. pET22B_PcrA_2Bchimera

   Plasmid

   #102998

   Purpose

   Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

   Depositor

   Martin Webb

   Insert

   PcrA_2Bchimera
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Bacilllus stearothermophilus PcrA in which the 2B…

   Promoter

   T7

   Available since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sperm whale Mb 64Y68F in pVP80K

   Plasmid

   #86956

   Purpose

   Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

   Depositor

   John Olson

   Insert

   Mb 64Y68F
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   64Y68F

   Promoter

   T5

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOX + hSlo2.2

   Plasmid

   #16215

   Depositor

   Larry Salkoff

   Insert

   KCNT1 potassium channel, subfamily (KCNT1 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   This construct lacks the first 53aa when compared…

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pBrg1-GGSG_AID_3xF_PP Target2

   Plasmid

   #235523

   Purpose

   Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed PgkPuro

   Depositor

   Brian Hendrich

   Insert

   Smarca4 (Smarca4 Mouse)
   

   Use

   Mouse Targeting

   Tags

   AID, 3xFLAG

   Expression

   Mutation

   None

   Promoter

   PGK

   Available since

   May 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZP05

   Plasmid

   #232319

   Purpose

   pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum

   Depositor

   Chenggang Wu

   Insert

   Ori-repA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10

   Plasmid

   #219699

   Purpose

   Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag

   Depositor

   Jennifer Nemhauser

   Insert

   pSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS415 GPDpro-RNQ1-CFP

   Plasmid

   #224887

   Purpose

   Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).

   Depositor

   Paul Herman

   Insert

   RNQ1 (RNQ1 Budding Yeast)
   

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-6p1-GST-Ubiquitin

   Plasmid

   #223236

   Purpose

   Bacterial expression construct for GST-Tagged Ubiquitin production and purification

   Depositor

   Weixing Zhao

   Insert

   Ubiquitin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pANY4-pR18/pL18-ccdB

   Plasmid

   #164899

   Purpose

   Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.

   Depositor

   Yingfeng An

   Insert

   ccdB cassette
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCASCADE-udhA-zwf

    Plasmid

    #87153

    Purpose

    udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    udhA-zwf guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid

    #100936

    Purpose

    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. NINJA TC

    Plasmid

    #159274

    Purpose

    Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

    Depositor

    Nikhil Joshi

    Article

    Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    NINJA TC
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. single polypeptide FPX biosensor for caspase-3

    Plasmid

    #60883

    Purpose

    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-3
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid

    #100941

    Purpose

    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
    

    Use

    Tags

    1xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

    Plasmid

    #100944

    Purpose

    Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    F636A, E660A, E725A, R295A

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. FGFR1_HUMAN_D0

    Plasmid

    #79719

    Purpose

    This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. LLP249 pGK-dCas9-DNMT3a V3

    Plasmid

    #100938

    Purpose

    dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. RANLS-LEHD-BNES

    Plasmid

    #60973

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    Caspase9 substrate sequence (LEHD) is after NLS s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. 1D3 HC

    Plasmid

    #170670

    Purpose

    Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)

    Depositor

    Ronald Jemmerson, Rafel Radi

    Article

    Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

    Insert

    HC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. 1D3 LC

    Plasmid

    #170669

    Purpose

    Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)

    Depositor

    Ronald Jemmerson, Rafel Radi

    Article

    Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

    Insert

    LC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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