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LXSN-Cx43-S279/282A

Plasmid

#60713

Purpose

retroviral expression of rat Cx43 S279/282A mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S279/282A

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S279/282D

Plasmid

#60715

Purpose

retroviral expression of rat Cx43 S279/282D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S279/282D

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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mIFP-Vimentin-7

Plasmid

#56242

Purpose

Localization: Intermediate Filaments, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Vimentin (VIM Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

Plasmid

#203841

Purpose

non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Mutation

G144A, Y145A, G146A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-EPHX1

Plasmid

#53114

Purpose

In vitro translation of microsomal epoxide hydrolase

Depositor

Xu Lin

Article

Chen et al BMC Cancer. 2014 Apr 23;14:282. doi: 10.

Insert

EPHX1 (EPHX1 Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA Flag-RARalpha (T181A,S445A,S461A)

Plasmid

#35556

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

Flag and His

Expression

Mammalian

Mutation

T181A, S445A, S461A

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
nes1852tk/lacZ

Plasmid

#47613

Purpose

used to create transgenic mice expressing LacZ reporter with Nestin enhancer

Depositor

Urban Lendahl

Article

Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

Insert

nestin 2nd intron (NES Human)

Use

Enhancer reporter

Tags

HSV tk promoter and lacZ

Mutation

2nd intron only

Promoter

HSV tk promoter

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP TEM4 DEL 125-135

Plasmid

#58896

Purpose

Expresses GFP tagged full length TEM4 protein with amino acids 125 - 135 deleted

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deleted aa's 125-135

Promoter

CMV

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pEBTetDBl-CLIP-α-tubulin

Plasmid

#136841

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (830-839)

Plasmid

#188091

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (830-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-hA1-BE3

Plasmid

#113418

Purpose

Expresses hA1-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA1-BE3 (APOBEC1 Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-hDDB2-R273H-V5

Plasmid

#167469

Purpose

ectopic expression of hDDB2 in mammalian cells

Depositor

Steven Artandi

Article

Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

Insert

DDB2 (DDB2 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

R273H

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-A2-77p-Venus

Plasmid

#64856

Purpose

Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

Depositor

Sriram Neelamegham

Article

Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

Insert

VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Human, Synthetic)

Tags

7X His and Venus

Expression

Bacterial

Promoter

T7 Promoter

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_EEF1A1_GTP-EFTU-D2

Plasmid

#109811

Purpose

Protein expression and purification of EEF1A1_GTP-EFTU-D2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A1_GTP-EFTU-D2 (EEF1A1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LXSN-Cx43-mAPPLE

Plasmid

#60696

Purpose

retroviral expression of rat Cx43 fused to mAPPLE

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

mAPPLE

Expression

Mammalian

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_GPR160

Plasmid

#45274

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

GPR160 (GPR160 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S279D

Plasmid

#60707

Purpose

retroviral expression of rat Cx43 S279D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S279D

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S282A

Plasmid

#60709

Purpose

retroviral expression of rat Cx43 S282A mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S282A

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-S282D

Plasmid

#60711

Purpose

retroviral expression of rat Cx43 S282D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S282D

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only