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Plasmid#70112PurposeExpression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomesDepositorInsertlyso-pHoenix (Cd63 Rat, Mouse, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
TagsArch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…ExpressionMammalianPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pN1-GCaMP6m-XC
Plasmid#111543PurposeImproved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-XDepositorInsertGCaMP6m-XC
ExpressionMammalianAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-STAT6-IRES-Neo
Plasmid#35482DepositorAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUmGW
Plasmid#22479DepositorInsertmembrane-bound EGFP
UseLentiviralTagsPalmitoylation signalExpressionMammalianAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ178 - cxTi10882_MCS
Plasmid#19331DepositorPublicationInsertcxTi10882 targetting region
ExpressionWormAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pR26 CAG/GFP Dest
Plasmid#74281PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseMouse TargetingAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSynSRE-T-Luc
Plasmid#60444PurposeUses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.DepositorInsertHMG-CoA synthase promoter (-324/-225)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterHMG-CoA synthase (-324/-225) & TATAA (-28/+39)Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-double floxed-eNpHR-EYFP-WPRE-pA
Plasmid#20949PurposeCre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibitionDepositorHas serviceAAV9Inserthalorhodopsin
UseAAVTagsEYFPExpressionMammalianPromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#50943PurposeBicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6f
UseAAVPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
TagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFV3
Plasmid#92072PurposeEmpty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.DepositorTypeEmpty backboneUseEmpty expression plasmid, for run-off transcripti…Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pECE
Plasmid#26453DepositorPublicationTypeEmpty backboneExpressionMammalianAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET22b RNase A
Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
TagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Rab5a-pmCherryC1
Plasmid#27679DepositorAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ230 (LbCpf1)
Plasmid#86210PurposeLbCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPR; Gateway compatible lbcpf1 entry cloneTags5' and 3' NLSExpressionPlantAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-myc-RhoA-T19N
Plasmid#12963DepositorAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDG459
Plasmid#100901PurposeSpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPRyl
Plasmid#70007PurposeCRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertionDepositorPublicationInsertsCodon optimized Cas9 from S. pyogenes
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-HDAC4
Plasmid#45636DepositorAvailable sinceJune 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
896 pcDNA3 T7 Akt1
Plasmid#9003DepositorInsertAkt1 (AKT1 Human)
ExpressionMammalianAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by