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pNCST-AvicFP3

Plasmid

#129506

Purpose

Expresses AvicFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-H5_Vietnam_1203/04_sfGFP_ST

Plasmid

#182546

Purpose

Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus

Depositor

Robert de Vries

Article

Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

Insert

H5_Vietnam_1203_04

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-DCX-TurboID-V5-puro

Plasmid

#190742

Purpose

A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

DCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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PLK3 1-334-pEGFP

Plasmid

#23266

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Tags

pEGFP

Expression

Mammalian

Mutation

aa 1-334

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-WT

Plasmid

#78920

Purpose

Mammalian expression of RNF138 with an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
GB1-A11(2-196, G175R)-Strep

Plasmid

#196211

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G175R

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G189E)-Strep

Plasmid

#196212

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G189E

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wNIR-GECO2-T2A-HO1

Plasmid

#159606

Purpose

Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

wNIR-GECO2-T2A-HO1

Expression

Worm

Promoter

tag-168

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-Ago2

Plasmid

#74231

Purpose

GFP-Ago2 lentiviral vector

Depositor

Carl Novina

Article

Janas et al RNA. 2012 Nov;18(11):2041-55. doi: 10.12

Insert

Ago2 (AGO2 Human)

Use

Lentiviral

Tags

eGFP

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-EFS-fDIO-GRAB_eCB2.0

Plasmid

#164610

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

EFS

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgNT

Plasmid

#138678

Purpose

Expresses a Non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Chicken Mermaid S188

Plasmid

#53617

Purpose

Genetically encoded voltage sensor based on mUKG-mKOk FRET pair

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken Mermaid S188

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCKC775

Plasmid

#226626

Purpose

piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
EF1a promoter, sfGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

7xTCF-LEF and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC764

Plasmid

#226625

Purpose

piggyBAC pCAG-hCas9-T2A-Unbiased3

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, S. pyogenes, Chicken)
EF1a promoter, blasticidin resistance

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC776

Plasmid

#226627

Purpose

piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
EF1a promoter, mCherry

Use

Synthetic Biology

Tags

oxygen dependent degron (ODD)

Expression

Mammalian

Promoter

4xHRE_YB TATA and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTet-GLI2shR

Plasmid

#136691

Purpose

Inducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5′CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3′)

Depositor

LuZhe Sun

Article

Zhu et al Lab Invest. 2018 Nov;98(11):1384-1396. d

Insert

shRNA_humanGli2 (GLI2 Human)

Use

Lentiviral

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFrt-invCAG-ires-Luc

Plasmid

#63576

Purpose

Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.

Depositor

Ivo Huijbers

Article

Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

Insert

Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG

Use

Cre/Lox; Frt/flpe recombination mediated insertion

Expression

Mammalian

Promoter

CAG

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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