We narrowed to 7,024 results for: 11

1. pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-AU1

   Plasmid

   #162096

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to ProtC-HA-AU1
   

   Use

   Lentiviral

   Tags

   ProtC-HA-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-ProtC

   Plasmid

   #162079

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-StrepTagII-ProtC
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-ProtC

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-HA

   Plasmid

   #162083

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-ProtC-HA
   

   Use

   Lentiviral

   Tags

   VSVg-ProtC-HA

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3/hArf5(WT)-mCherry

   Plasmid

   #79421

   Purpose

   Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf5 (ARF5 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX302_HOXB13

   Plasmid

   #70089

   Purpose

   Expression of human HOXB13 from CMV promoter, puromycin selection marker

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-vCre

   Plasmid

   #55638

   Purpose

   EF1a-driven expression of VCre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   vCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6/V5-DEST-RINT1

   Plasmid

   #40124

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   RINT1 (RINT1 Human)
   

   Use

   Lentiviral

   Tags

   V5 epitope

   Expression

   Mammalian

   Mutation

   L270M relative to NM_021930

   Promoter

   CMV

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3XFLAG-CMV-14-WDR5

   Plasmid

   #59974

   Purpose

   Mammalian Expression of WDR5-FLAG

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEVOL-pAzF[TAG]

   Plasmid

   #164579

   Purpose

   Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Inserts

   Mj pCNFRS[TAG] (aaRS1)
   Mj pCNFRS[TAG] (aaRS2)
   Mj tRNA[TAG]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   araBAD, glnS, and proK

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid

    #140196

    Purpose

    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    

    Use

    CRISPR and Synthetic Biology; Fungal genome integ…

    Tags

    VPR (VP64-p65-Rta) , NLS

    Expression

    Mutation

    Promoter

    PgpdA and PtrpC

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-Blast-sgAMPKa1

    Plasmid

    #138704

    Purpose

    Expresses a human AMPKa1-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgAMPKa1 human (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Puro-sgAMPKa2

    Plasmid

    #138685

    Purpose

    Expresses a human AMPKa2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgAMPKa2 human (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFL His-p97

    Plasmid

    #113510

    Purpose

    Expression of His-p97 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. DRH296: FCK-Optopatch2

    Plasmid

    #51694

    Purpose

    Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    Optopatch2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHis-LMW_FGF2

    Plasmid

    #157657

    Purpose

    Expresses FGF2 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changed Cystein 211,229 to serine, deleted amino …

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGreenFire_blastocidin

    Plasmid

    #174103

    Purpose

    pGreenFire MPRA plasmid for cloning

    Depositor

    Paul Khavari

    Article

    Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FCK-ChrimsonR-GFP

    Plasmid

    #59049

    Purpose

    Lentiviral expression of ChrimsonR under the CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    CaMKII

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE-mCardinal-P2A-B19L Flp-in vector

    Plasmid

    #178519

    Purpose

    For making TRE-CB mouse line in Tigre locus

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mCardinal-P2A-B19L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid

    #71828

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid

    #118586

    Purpose

    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

    Depositor

    Radhakrishnan Padmanabhan

    Article

    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

    Insert

    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    

    Use

    Yeast-e. col shuttle vector prs424

    Tags

    Expression

    Mutation

    The conserved GDD in the NS5 polymerase gene muta…

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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