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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,359 results for: cas9

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Showing: 4701 - 4720 of 14359 results

  3. SpCas9-NRCH-YE1-BE4max

    Plasmid
    #198549
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198549-plasmid-map-sequence-id-388903

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  4. HiFi-nCas9-NG_P2A-mCherry

    Plasmid
    #219936
    Purpose
    Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems
    Depositor
    Alexis Komor
    Publication
    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
    Use
    CRISPR
    Tags
    SV40 bpNLS and SV40 bpNLS-P2A-mCherry
    Expression
    Mammalian
    Mutation
    HiFi-nCas9(D10A/R691A)
    Promoter
    CMV
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219936-plasmid-map-sequence-id-435504

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  7. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid
    #133316
    Purpose
    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas9 (Streptococcus pyogenes)
    Use
    CRISPR
    Promoter
    xylose
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133316-plasmid-map-sequence-id-266825

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Showing: 4701 - 4720 of 14359 results