We narrowed to 13,665 results for: crispr cas9

1. pDIV270

   Plasmid

   #226181

   Purpose

   Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p (Komagataella phaffii)

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB578

   Plasmid

   #141095

   Purpose

   E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Inserts

   Cas9 and tracrRNA
   repeat-spacer-repeat array
   

   Expression

   Bacterial

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TetO-FUW-VdC9BV

   Plasmid

   #62195

   Purpose

   Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

   Depositor

   Kam Leong

   Article

   Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

   Insert

   VP64dCas9BFPVP64
   

   Use

   Lentiviral

   Tags

   Two VP64s tagged to dCas9 fused to BFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive) Cas9 (dCas9)

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRB14

   Plasmid

   #52522

   Purpose

   expresses a myc-tagged version of hCas9 in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   S. pyogenes cas9 with humanized codon bias
   

   Tags

   myc

   Expression

   Insect

   Promoter

   tubulin

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3TS-zSpG

   Plasmid

   #179316

   Purpose

   In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpG
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-zSpRY

   Plasmid

   #179317

   Purpose

   In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Zebrafish codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR; In vitro transcription by t3 promoter

   Tags

   SV40-NLS

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW014-2

   Plasmid

   #85612

   Purpose

   Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   ugtP-gRNA.P395T
   

   Expression

   Bacterial

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC67895

   Plasmid

   #209506

   Purpose

   Cloning protospacers for Cas12a editing

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas12a-tRNA-DR-DR
   

   Use

   CRISPR

   Promoter

   TaU6

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LRG2.1-TagBFP2

   Plasmid

   #124773

   Purpose

   Lentiviral plasmid to co-express a guide RNA and TagBFP2

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. psLIB CMV

    Plasmid

    #229953

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW019-2

    Plasmid

    #85614

    Purpose

    Inducible dCas9 integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    dcas9
    

    Expression

    Bacterial

    Promoter

    PxylA (B. megaterium)

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC67897

    Plasmid

    #209491

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EC67899

    Plasmid

    #209493

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EC70130

    Plasmid

    #209501

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Promoter

    TaU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HPRT2b-pGEM

    Plasmid

    #69539

    Purpose

    gRNA targeting human HPRT gene

    Depositor

    Hong Yan

    Article

    Liao et al Nucleic Acids Res. 2015 Jun 29. pii: gkv

    Insert

    HPRT gRNA2
    

    Expression

    Mammalian

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. EC67894

    Plasmid

    #209505

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Promoter

    TaU6

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJAT74

    Plasmid

    #204296

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. EC67892

    Plasmid

    #209499

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Promoter

    TaU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EC67908

    Plasmid

    #211795

    Purpose

    Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    HptII, Cas12a, GR-GRF-GIF
    

    Expression

    Plant

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX-sgRNA-BfuAI-2k

    Plasmid

    #112915

    Purpose

    Empty sgRNA expression plasmid

    Depositor

    Ren-Jang Lin

    Article

    Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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