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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194723

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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SAFB1-EGFP

Plasmid

#32746

Depositor

Steffi Oesterreich

Article

Townson et al Clin Cancer Res. 2000 Sep;6(9):3788-96.

Insert

SAFB1 (SAFB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pUBC_V5_MS2-mCherry

Plasmid

#68420

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

V5 and mCherry

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

human UbC

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Cdc42

Plasmid

#180577

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHLUM (version 2)

Plasmid

#64166

Purpose

harbours marker genes HIS3, LEU2, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag7

Plasmid

#175533

Purpose

Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA-Venus

Plasmid

#186372

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-PuroTK-L2

Plasmid

#141005

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

Plasmid

#55219

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAG364-greenFAST

Plasmid

#160353

Purpose

expresses greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

greenFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-THT

Plasmid

#55790

Purpose

GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Type

Empty backbone

Use

RNAi; Gateway

Expression

Mammalian

Promoter

modified H1 RNA gene

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSV2 neuNT

Plasmid

#10918

Depositor

Bob Weinberg

Article

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuNT (Erbb2 Rat)

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only
pAG365-redFAST

Plasmid

#160354

Purpose

Expresses redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES

Plasmid

#196828

Purpose

Express dpdpCas13b with AAAA mutation

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

dpspCas13b(AAAA)

Tags

3XFLAG and NES

Expression

Mammalian

Mutation

K367A, D369A and K370A

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxOFP

Plasmid

#229950

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

Plasmid

#244217

Purpose

Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-mnx1

Plasmid

#82402

Purpose

Gateway-compatible zebrafish mnx1 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mnx1 promoter

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJJB1439

Plasmid

#218589

Purpose

Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

GES

Tags

ePTS1

Expression

Yeast

Mutation

Erg20(F96W, N127W)

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-HaloTag9

Plasmid

#175546

Purpose

Expression of HaloTag9 at the Golgi apparatus of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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