We narrowed to 10,059 results for: Uty

1. Prig-3::CD4::spGFP11

   Plasmid

   #65830

   Purpose

   For membrane contact

   Depositor

   Kang Shen, Cori Bargmann

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
   

   Tags

   spGFP11

   Expression

   Worm

   Promoter

   Prig-3

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMP71-tCD19-mGFP

   Plasmid

   #174609

   Purpose

   expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   CD19 extracellular and transmembrane domain (Cd19 Mouse)
   

   Tags

   palmitoylated EGFP

   Expression

   Mammalian

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME map2k2a_s219D

   Plasmid

   #201058

   Purpose

   Gateway middle entry vector expressing constitutively active zebrafish map2k2a

   Depositor

   Sarah Childs

   Article

   Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

   Insert

   MAP2k2a (map2k2a Zebrafish)
   

   Use

   Zebrafish expression

   Mutation

   Mutation S219D, constitutively active

   Promoter

   none

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCST-AausFP1

   Plasmid

   #129499

   Purpose

   Expresses AausFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP1
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-erExRai-AKAR2

   Plasmid

   #161756

   Purpose

   ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   CYP450-ExRai-AKAR2
   

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAnc80L65

   Plasmid

   #68837

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   Ancestral AAV Capsid Anc80L65
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   Rep2

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-ExRai-AKAR2(T/A)

   Plasmid

   #161754

   Purpose

   Negative-control mutant for ExRai-AKAR2 biosensor.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2(T/A)
   

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MICU-APEX2

   Plasmid

   #79057

   Purpose

   A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   C-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Promoter

   CMV immearly promotor

   Available Since

   Sept. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCST-AvicFP1

   Plasmid

   #129504

   Purpose

   Expresses AvicFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP1
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNCST-AvicFP2

    Plasmid

    #129505

    Purpose

    Expresses AvicFP2 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP2
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNCST-AvicFP3

    Plasmid

    #129506

    Purpose

    Expresses AvicFP3 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP3
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFP5380 LAMP1-SBP- mCherry-FIREtag

    Plasmid

    #216725

    Purpose

    Expresses LAMP1-mCherry-FIREtag in mamallian cells

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    LAMP-1 (LAMP1 Human)
    

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEND-184_Cacnes_mCherry

    Plasmid

    #225610

    Purpose

    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    V5-6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEND-164_Cacnes_pFAST

    Plasmid

    #225616

    Purpose

    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    pFAST
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAuroraA-GFP-AURKA-mCherry

    Plasmid

    #99878

    Purpose

    Expression of GFP-AURKA-mCherry in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Promoter

    AURKA minimal

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2T-cmv-SpCas9-TadCBEd-BlastR

    Plasmid

    #193848

    Purpose

    Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRubiC-T2A-Cas9

    Plasmid

    #75347

    Purpose

    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    mCherry (via t2a)

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-TEX264-deltaLRR-His6

    Plasmid

    #220215

    Purpose

    Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    c.4-75del / p.2-24del

    Promoter

    T7

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-pTH-iCre-WPREpA

    Plasmid

    #213130

    Purpose

    Truncated rat TH promoter expressing iCre

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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