Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,770 results for: BLI;

Clear keywords
Showing: 4741 - 4760 of 9770 results

  1. pT7CFE1_EVD68_3ABC_C147R

    Plasmid
    #212978
    Purpose
    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed Cysteine 147 in 3C to Arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212978-plasmid-map-sequence-id-420358

    Enlarge

  2. pT7CFE1_EVD68_3ABCD_C147R

    Plasmid
    #212980
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed cysteine 147 from 3C to arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212980-plasmid-map-sequence-id-420353

    Enlarge

  3. pT7CFE1_EVD68_VP12A_N84T

    Plasmid
    #212983
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A N84T
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed Asparagine 84 from 2A to Threonine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212983-plasmid-map-sequence-id-420352

    Enlarge

  4. pET28-Flag-RBM24-1~93

    Plasmid
    #208633
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 1~93, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208633-plasmid-map-sequence-id-415921

    Enlarge

  5. pET28-RPL3-Twstrep

    Plasmid
    #208623
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) (RPL3 Human)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208623-plasmid-map-sequence-id-418316

    Enlarge

  6. pAMW-Wnk

    Plasmid
    #203978
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Tags
    6xMyc
    Expression
    Insect
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203978-plasmid-map-sequence-id-403206

    Enlarge

  7. XBB.1.5+R403K

    Plasmid
    #208586
    Purpose
    Mammalian expression vector for expressing XBB.1.5+R403K SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+R403K spike (S )
    Expression
    Mammalian
    Available Since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208586-plasmid-map-sequence-id-409585

    Enlarge

  8. XBB.1.5+F456L

    Plasmid
    #208585
    Purpose
    Mammalian expression vector for expressing XBB.1.5+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+F456L spike (S )
    Expression
    Mammalian
    Available Since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208585-plasmid-map-sequence-id-409584

    Enlarge

  9. XBB.1.5+K478R

    Plasmid
    #208587
    Purpose
    Mammalian expression vector for expressing XBB.1.5+K478R SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+K478R spike (S )
    Expression
    Mammalian
    Available Since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208587-plasmid-map-sequence-id-409578

    Enlarge

  10. pCDH-Flag-hS1pr1-S5A_Neo

    Plasmid
    #203710
    Purpose
    Overexpression of FLAG-S1PR1-S5A mutant.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1-S5A (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S5A
    Promoter
    CMV
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203710-plasmid-map-sequence-id-403867

    Enlarge

  11. pALuWBB

    Plasmid
    #183202
    Purpose
    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA
    Depositor
    Mitch McVey
    Insert
    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    Use
    CRISPR; Recombines at attp landing site in fly ge…
    Expression
    Insect
    Promoter
    U6-3
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    183202-plasmid-map-sequence-id-362157

    Enlarge

  12. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid
    #182894
    Purpose
    Mammalian expression of PVALB rat mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) heavy chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182894-plasmid-map-sequence-id-360571

    Enlarge

  13. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid
    #182895
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182895-plasmid-map-sequence-id-360643

    Enlarge

  14. TRE3GV-somBiPOLES-mCerulean

    Plasmid
    #192578
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192578-plasmid-map-sequence-id-379291

    Enlarge

  15. CaMKII-somBiPOLES-eGFP

    Plasmid
    #192583
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Promoter
    CaMKIIa(0.4)
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192583-plasmid-map-sequence-id-379280

    Enlarge

  16. pLenti-PGK-NXPH4 (neo)

    Plasmid
    #192078
    Purpose
    Lentivirus for expression of NXPH4
    Depositor
    Roland Friedel
    Insert
    NXPH4 (NXPH4 Human)
    Use
    Lentiviral
    Promoter
    hPGK
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192078-plasmid-map-sequence-id-379235

    Enlarge

  18. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

    Enlarge

  19. pB6-Rosa26-cre_dre_reporter_1_2

    Plasmid
    #185966
    Purpose
    B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Expression
    Mammalian
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185966-plasmid-map-sequence-id-367346

    Enlarge

  20. pB6-Rosa26-cre_dre_reporter_1_1b

    Plasmid
    #185965
    Purpose
    B6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Expression
    Mammalian
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185965-plasmid-map-sequence-id-367345

    Enlarge

Showing: 4741 - 4760 of 9770 results