We narrowed to 9,737 results for: Ada;

1. pT3TS-spCas9 ABE-Umax-ex1

   Plasmid

   #222141

   Purpose

   Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcs2+spCas9 ABE-Umax-nanos

   Plasmid

   #222142

   Purpose

   Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9 with nanos UTR
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   SP6

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHDR_NPM2-T2A-mGreenLantern-PuroTK

   Plasmid

   #222910

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHDR_FIGLA-T2A-mGreenLantern-PuroTK

   Plasmid

   #222905

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

   Plasmid

   #222915

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-PRDM1

   Plasmid

   #222924

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   PRDM1 (PRDM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-ZGLP1

   Plasmid

   #222926

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   ZGLP1 (ZGLP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERH-SOHLH1

   Plasmid

   #222922

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   SOHLH1 (SOHLH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT3TS-spCas9 ABE-Umax-ex2-rest1

   Plasmid

   #222143

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT3TS-spCas9 ABE-Umax-ex1-rest1

    Plasmid

    #222140

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. opt.a_pSTC0-zeo

    Plasmid

    #202347

    Purpose

    Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    opt.a
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. opt.b_pSTC0-zeo

    Plasmid

    #202348

    Purpose

    Design opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    opt.b
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. neg.ctrl_pSTC0-zeo

    Plasmid

    #202350

    Purpose

    Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    neg. ctrl
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Q54A

    Plasmid

    #217912

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP Q54A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Glutamine at position 55 and 267 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. T40A

    Plasmid

    #217911

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP T40A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Threonine at position 41 and 253 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJM166B

    Plasmid

    #218123

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-GCaMP6f-WPRE

    Plasmid

    #216275

    Purpose

    AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    human Synapsin 1 (hSyn)

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid

    #141237

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    Human elongation factor-1 alpha (EF-1 alpha)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

    Plasmid

    #208869

    Purpose

    For mammalian expression of NAP1 (delta-TBK1)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L226Q/L233Q (disrupts TBK1 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T1-GST-TEV-GFP-NAP1

    Plasmid

    #208870

    Purpose

    For expression in E. coli of GFP-NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    GFP and GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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