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p2T-CAG-SpCas9-BlastR

Plasmid

#107190

Purpose

Confers constitutive expression of SpCas9

Depositor

Richard Sherwood

Publication

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 (NEWENTRY )

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial and Mammalian

Promoter

Chicken ß-actin

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPro18-km

Plasmid

#17808

Depositor

Jay Keasling

Publication

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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pET-UbcH10-wt

Plasmid

#8503

Depositor

Joan Ruderman

Publication

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Tags

His

Expression

Bacterial

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFastBac-CHD7

Plasmid

#170141

Purpose

Expresses CHD7 in insect cells

Depositor

Robert Kingston

Publication

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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[pSK729] pRK5 HA-FLCN(R164A)

Plasmid

#136147

Purpose

mammalian expression of FLCN

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Cell. 2019 Nov 27;179(6):1319-1329.e8. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLCN (FLCN Human)

Tags

HA

Expression

Mammalian

Mutation

R164A

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-GB2

Plasmid

#125710

Purpose

Eukaryotic expression of the GB2 subunit of the metabotropic GABA receptor

Depositor

Kai Johnsson

Publication

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GB2

Expression

Mammalian

Promoter

CMV

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Publication

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-LBK1(K78I) (STK11 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+8CmCitrine

Plasmid

#34950

Depositor

Amro Hamdoun

Publication

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CBP-HAT-pET

Plasmid

#21091

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Tags

His and T7 tag

Expression

Bacterial

Mutation

HAT domain aa 1171-1621

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pOSIP-KH-RBS2-dCas9

Plasmid

#182740

Purpose

Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

Depositor

David Bikard

Publication

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Insert

dCas9

Use

CRISPR; Integrative vector

Promoter

Ptet

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-CFL-SN

Plasmid

#181740

Purpose

AAV vector for expressing CFL-SN

Depositor

Yasunori Hayashi

Publication

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

CFL-SN

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCFP

Plasmid

#28011

Depositor

Lingchong You

Publication

Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

Insert

CFP

Expression

Bacterial

Available since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pBetaActin-mNeonGreen-CAMSAP3

Plasmid

#191322

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Publication

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNeonGreen-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDBTrp-LexABD-CRY2FL

Plasmid

#78210

Purpose

Use with Addgene 40615 for blue light transcription control in yeast

Depositor

Chandra Tucker

Publication

Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

Insert

LexABD-CRY2 (CRY2 Mustard Weed)

Tags

lexA binding domain

Expression

Yeast

Mutation

contains endogenous CRY2 NLS

Promoter

ADH1

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-p58

Plasmid

#166942

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

Depositor

Jennifer Lippincott-Schwartz

Publication

Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p58 (Lman1 Rat)

Tags

GFP and chicken lysozyme signal peptide

Expression

Mammalian

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFCIV-CEACAM6

Plasmid

#149303

Purpose

lentiviral packaging vector for CEACAM6 expression.

Depositor

James Fleckenstein

Publication

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

ubiquitin C

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_CEACAM6-His

Plasmid

#149337

Purpose

for eukaryotic expression and purification of CEACAM6-His6

Depositor

James Fleckenstein

Publication

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Tags

IL-2 signal peptide and polyhistidine

Expression

Mammalian

Mutation

point mutation (GGA to GCA) to replace 'G-g…

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR FL CPAP RNAi resistant

Plasmid

#46390

Depositor

Pierre Gonczy

Publication

Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

Insert

CPAP RNAi resistant mutant (CENPJ Human)

Use

Gateway Cloning

Mutation

silent mutation that renders it resistant to siRN…

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only
pNO41

Plasmid

#101067

Purpose

BphP1-PpsR2 chromophore plasmid; ho1

Depositor

Jeffrey Tabor

Publication

Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

Insert

ho1

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-RPA43

Plasmid

#17659

Depositor

Tom Misteli

Publication

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RPA43 (Twistnb Mouse)

Tags

EGFP

Expression

Mammalian

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles