We narrowed to 48,883 results for: des.1

1. pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204214

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DNA binding domain (delDBD)

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204191

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of R162

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

   Plasmid

   #204213

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Point mutation on I207M

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204211

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Point mutation on Q175* (*termination)

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204210

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Point mutation on R178* (*termination)

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

   Plasmid

   #204212

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NK2 homeobox 1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Point mutation on I207F

   Expression

   Mammalian

   Mutation

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

   Plasmid

   #193719

   Purpose

   Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
   

   Use

   Tags

   2xHA-FRB

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

   Plasmid

   #175051

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Thr76 to Asp, phosphomimetic mutation

   Promoter

   T7 promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

   Plasmid

   #175050

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75240

    Purpose

    CRISPR/Cas9 plasmid against human Ikaros

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

    Plasmid

    #122982

    Purpose

    AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    mCh-IRES-BoNT/B(1-146)-iLID
    

    Use

    AAV

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    iLID contains long lived V416I mutation

    Promoter

    human synapsin

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75244

    Purpose

    CRISPR/Cas9 plasmid against human Helios

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Helios (IKZF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)

    Plasmid

    #223778

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Insect

    Mutation

    W18A/L21A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR)

    Plasmid

    #223748

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    W36A/L39A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-3_Flag-YTHDF2-D-MUT (nonsense mutation of sgYTHDF2-1)

    Plasmid

    #232947

    Purpose

    Expresses the domain-mutant YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2-D-MUT (YTHDF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    5 site mutation (Y418A+D422A+W432A+W486A+W491A), …

    Promoter

    Available since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3)

    Plasmid

    #223775

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    I224A/L227A; deletion of LIR3

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4)

    Plasmid

    #223776

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    I307A/V310A; deletion of LIR4

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A; deltaLIR2)

    Plasmid

    #223783

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Y213A/I216A; deletion of LIR2

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A; deltaLIR1)

    Plasmid

    #223746

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    W276A/I279A; deletion of LIR1

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (Y18A/L21A; deltaLIR)

    Plasmid

    #223750

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    FUNDC1 soluble part (FUNDC1 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    expresses aa 1-50, with Y18A/L21A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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