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pEXP5-NT-7th R

Plasmid

#42573

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated calmodulin gene

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pM148: pAAV.CMV-Cas13e-NT sgRNA

Plasmid

#203446

Purpose

Plasmid expressing active RfxCas13d with non-targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-non targeting (NT) sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Blast NT shRNA

Plasmid

#123306

Purpose

Expression of a non-targeting shRNA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

Non-targeting shRNA

Use

Lentiviral and RNAi

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS2702-CMV-iNme2-ABE8e-nt

Plasmid

#228723

Purpose

Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with iNme2 nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2703-CMV-iNme2Smu-ABE8e-nt

Plasmid

#228724

Purpose

Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with iNme2Smu nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

Plasmid

#163169

Purpose

Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

Non-targeting

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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PCLP-2/NT-IgG (104)

Plasmid

#24423

Depositor

Steven Rosen

Article

Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

Insert

PCLP-2 (PODXL2 Human)

Tags

Human IgG1 Fc

Expression

Mammalian

Mutation

N-terminal (acidic) domain of PCLP-2 (1-630 bp) f…

Available Since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only
pD(Nt)_His6-GST-TEV

Plasmid

#223878

Purpose

4G-cloning donor for N-terminal His6-GST-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-GST-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-MBP-TEV

Plasmid

#223877

Purpose

4G-cloning donor for N-terminal His6-MBP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-MBP-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-HA2-TEV

Plasmid

#223876

Purpose

4G-cloning donor for N-terminal His6-HA2-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-HA2-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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