Addgene

We narrowed to 1,225 results for: SUMO

Showing: 691 - 720 of 1225 results

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pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

Plasmid

#221447

Purpose

Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

SPR degron: hGEM (1-110) fragment that is fused t…

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-EGFP-N

Plasmid

#210270

Purpose

N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 RPS6KL1 MIT, residues 20-140

Plasmid

#180623

Purpose

Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

RPS6KL1 (RPS6KL1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 20-140

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SNX15 MIT residues 263-342

Plasmid

#180625

Purpose

Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SNX15 (SNX15 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 263-342

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , F124D, residues 108-200

Plasmid

#180629

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, F124D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , L177D, residues 108-200

Plasmid

#180634

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, L177D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP8, residues 1-147

Plasmid

#180636

Purpose

Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP8 (USP8 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-147

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165

Plasmid

#180611

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, V18D

Plasmid

#180612

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, V18D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, F98D

Plasmid

#180613

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, F98D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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hHSF4b K294R

Plasmid

#118357

Purpose

This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Use

Tags

Expression

Mammalian

Mutation

K294R

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Gal4-HSF4b WT

Plasmid

#118358

Purpose

This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB24

Plasmid

#91959

Purpose

empty expression vector for 10His-SUMO tag protein purification

Depositor

Christopher Bahl

Type

Empty backbone

Use

Tags

10-His and Smt3

Expression

Bacterial and Yeast

Mutation

Promoter

T7

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDG04135

Plasmid

#196013

Purpose

Nanobody Zasp-Nano65 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ZaspNano65-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04136

Plasmid

#196014

Purpose

Nanobody Zasp-Nano66 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ZaspNano66-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04095

Plasmid

#196006

Purpose

Nanobody Obs-Nano56 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ObsNano56-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG03247

Plasmid

#195993

Purpose

Nanobody Sls-Nano42 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- SlsNano42-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04092

Plasmid

#196009

Purpose

Nanobody Obs-Nano59 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ObsNano59-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04110

Plasmid

#196012

Purpose

Nanobody Actn-Nano64 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ActnNano64-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04093

Plasmid

#196005

Purpose

Nanobody Obs-Nano55 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ObsNano55-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04091

Plasmid

#196008

Purpose

Nanobody Obs-Nano58 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ObsNano58-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04108

Plasmid

#196010

Purpose

Nanobody Actn-Nano62 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ActnNano62-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04096

Plasmid

#196007

Purpose

Nanobody Obs-Nano57 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ObsNano57-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG03246

Plasmid

#195997

Purpose

Nanobody Proj-Nano29 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ProjNano29-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDG03248

Plasmid

#195991

Purpose

Nanobody Sls-Nano2 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- SlsNano2-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04109

Plasmid

#196011

Purpose

Nanobody Actn-Nano63 production

Depositor

Dirk Görlich

Insert

H14-SUMO-Cys- ActnNano63-Cys

Use

E.coli expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hMEF2A K403R

Plasmid

#118355

Purpose

This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K403R

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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MZB131

Plasmid

#217839

Purpose

Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

AP3D1 (AP3D1 Human)

Use

Tags

10x His, HaloTag, and SUMO (SMT3)

Expression

Bacterial

Mutation

Deleted residues 1-919

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MZB150

Plasmid

#217840

Purpose

Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

ARNO (CYTH2 Human)

Use

Tags

10x His and SUMO (SMT3)

Expression

Bacterial

Mutation

Deleted residues 1-51 and 253-400

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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