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Showing: 921 - 960 of 1444 results

  1. SCN1Aprom(1-500)-H2B-GFP

    Plasmid
    #236162
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 500 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236162-plasmid-map-sequence-id-468412

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  2. SCN1Aprom(335-406)-H2B-GFP

    Plasmid
    #236151
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236151-plasmid-map-sequence-id-468384

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  4. EW416 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmLink-ZIK1KRAB

    Plasmid
    #236140
    Purpose
    Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-ZIK1KRAB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236140-plasmid-map-sequence-id-468371

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  5. EW425 CMV-TO-ZNF35(5-8)-IKZF1(2-3)-realDeImmLink-ZIK1KRAB

    Plasmid
    #236142
    Purpose
    Plasmid encoding the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ZNF35(5-8)-IKZF1(2-3)-deImmunLink-ZIK1KRAB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236142-plasmid-map-sequence-id-468373

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  15. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

    Plasmid
    #205603
    Purpose
    Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion
    Depositor
    Tarun Kapoor
    Article
    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410
    Insert
    γ-tubulinWT-RNAi-resistant-GFP
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    G2228A, C2231T, C2237T, G2240A
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205603-plasmid-map-sequence-id-405874

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  17. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111431-plasmid-map-sequence-id-292191

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  30. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

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  32. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Article
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Expression
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    62192-plasmid-map-sequence-id-156364

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  33. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid
    #52890
    Purpose
    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.
    Depositor
    Iswar Hariharan
    Article
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Inserts
    LexGAD
    Gal4 (GAL4 Budding Yeast)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    52890-plasmid-map-sequence-id-211914

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  37. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174647
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    CMV (with TetOn)
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174647-plasmid-map-sequence-id-345296

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  40. pcDNA5/FRT/TO/SH/GW-Mst1

    Plasmid
    #84299
    Purpose
    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.
    Depositor
    Jonathan Chernoff
    Article
    Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71
    Insert
    Mst1 (STK4 Human)
    Use
    Tags
    HA, SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 921 - 960 of 1444 results