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Hy_pMT Laccase2 MCS-HIPK3 Exon

Plasmid

#69888

Purpose

Expresses the human HIPK3 exon 2 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Fly, Human)

Expression

Insect

Mutation

Expresses aa1-194 of HIPK3

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBacPak-HA-Non-stop

Plasmid

#52289

Purpose

Expresses Drosophila Non-stop

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Non-stop (not Fly)

Tags

HA

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.BRWD3-V5H

Plasmid

#48624

Purpose

Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cells

Depositor

Aziz Sancar

Article

Ozturk et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

BRWD3 (BRWD3 Fly)

Tags

V5 and His tags

Expression

Mammalian

Mutation

no mutations, stop was removed to fuse V5His at C…

Promoter

CMV

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
Donor_C_FH_VIR

Plasmid

#186660

Purpose

Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Shu

Plasmid

#186655

Purpose

Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_Nbr

Plasmid

#186662

Purpose

Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_N_FH_GLE

Plasmid

#186657

Purpose

Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

Plasmid

#112987

Purpose

In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Mutation

S123-125-3A

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

Plasmid

#112985

Purpose

In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

Insert

mei-S332 (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Mutation

S123-125-3D

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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UASp-deltaN cycB

Plasmid

#112967

Purpose

P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Lee et al Genetics. 2001 Aug;158(4):1545-56.

Insert

cycB (CycB Fly)

Use

P insertion vector

Mutation

N-term deletion of first 46 amino acids, includes…

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pnos-Cas9-nos

Plasmid

#62208

Purpose

Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

nanos

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pUAS-PE2-attB

Plasmid

#149550

Purpose

Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Expression

Insect

Promoter

UAS

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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KHBD00727

Plasmid

#39748

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9932 (CG9932 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pMT-Diaphanous-GFP

Plasmid

#24287

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Diaphanous (dia Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
dBrainbow

Plasmid

#29327

Depositor

Julie Simpson

Article

Hampel et al Nat Methods. 2011 Mar;8(3):253-9. doi: 1

Insert

UAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5

Expression

Insect

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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KHBD00222

Plasmid

#34370

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1832 (CG1832 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only