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Plasmid#82354PurposeT7 in vitro transcription template for Bas mRNADepositorInsertBAS1509 hypothetical protein
UseUnspecifiedTagsExpressionMutationPromoterT7Available sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-AqpZ-ALFA
Plasmid#212310PurposeExpression of AqpZ with ALFA tagDepositorInsertAquaporin Z
UseTagsALFA tag and Strep-Tag IIExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-Sir2
Plasmid#51742Purposeoverexpression of Sir2 in S. cerevisiae, HIS3 markerDepositorInsertSir2 (SIR2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
p414ADH_Hsp82_CycTER
Plasmid#61712Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterADH1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-STX6
Plasmid#31581DepositorInsertsyntaxin 6 (STX6 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
p414TEF_Hsp82_CycTER
Plasmid#61711PurposeYeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterTEF1Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
T1ACR1_EYFP_pcDNA3.1
Plasmid#161027PurposeExpresses strongly red-shifted T1ACR1 in mammalian cellsDepositorInsertT1ACR1
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIM35
Plasmid#74644PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoterMET25Available sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-HST2
Plasmid#51745Purposeoverexpression of HST2 in S. cerevisiae, HIS3 markerDepositorInsertHST2 (HST2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
p414GPD_Hsp82_CycTER
Plasmid#61710PurposeYeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterGPDAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Sap
Plasmid#206856PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_URA
Plasmid#206848PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1061
Plasmid#209550PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA geneDepositorInsertE2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only