Addgene

We narrowed to 7,579 results for: Lif

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pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

Plasmid

#163928

Purpose

Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

Plasmid

#124066

Purpose

E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-R232Q-sfGFP

Use

Tags

sfGFP

Expression

Bacterial

Mutation

R232Q

Promoter

trc

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-ch-TOG-His6

Plasmid

#69108

Purpose

For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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CLXSN 5myc-CDC6-WT

Plasmid

#109332

Purpose

express tagged Cdc6 WT

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast-CDT1-HA

Plasmid

#109335

Purpose

virus packaged, doxycyline inducible expression of Cdt1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cdt1 (CDT1 Human)

Use

Lentiviral; Inducible expression

Tags

HA, His, Strep

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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BCL9-BirA*

Plasmid

#109147

Purpose

To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

Depositor

Mariann Bienz

Article

van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

Insert

BCL9 (BCL9 Human)

Use

Tags

BirA(R118G)

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-GTSE1(long)-His6

Plasmid

#69110

Purpose

For bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

G2- and S-phase Expressed 1 (GTSE1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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p1.2-GS

Plasmid

#162770

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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B9L-BirA*

Plasmid

#109146

Purpose

To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

Depositor

Mariann Bienz

Article

van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

Insert

B9L (Bcl9l Mouse)

Use

Tags

BirA(R118G) and Flag

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-TBX20a

Plasmid

#98620

Purpose

Gateway entry TOPO TA cloning vector for TBX20a

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Tbx20a (Tbx20 Mouse)

Use

Topo ta cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPSt_35Spr-GFP-t35S

Plasmid

#203592

Purpose

Fully assembled Plant STARR-seq plasmid with the 35S terminator.

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Inserts

CaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMKRQ_11_DExCon-mNeonGreen-R25

Plasmid

#179896

Purpose

Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB25, member RAS oncogene family (RAB25 Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5 Y191C

Plasmid

#98613

Purpose

Gateway entry vector for NKX2-5 Y191C

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 Y191C (Nkx2-5 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Point mutation Y191C

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DHFR.cHSF1

Plasmid

#134739

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ERF

Plasmid

#98626

Purpose

Gateway entry TOPO TA cloning vector ERF

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ERF (Erf Mouse)

Use

Topo ta cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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CLXSN 5myc-CDC6-mut

Plasmid

#109333

Purpose

express tagged Cdc6 mutant

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

R56A, L59A, K81A, E82A, N83A

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-flex-TVA

Plasmid

#69618

Purpose

Express recepter TVA for envA with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Insert

Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)

Use

AAV and Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA-gp78-FLAG RING mutant C356G H361A

Plasmid

#61751

Purpose

express C-terminally FLAG tagged human gp78

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

gp78 (AMFR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C356G H361A; P179L, F262L, Y315C, and D346G (ple…

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pENTR-2B-Gata4

Plasmid

#98615

Purpose

Gateway entry vector for Gata4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Gata4 (Gata4 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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