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We narrowed to 1,350 results for: RPL

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Showing: 461 - 480 of 1350 results

  1. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  2. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  4. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  8. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  10. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid
    #208636
    Purpose
    for E. coli expression of human eIF5B (ENST00000289371)
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    208636-plasmid-map-sequence-id-433645

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  11. pcDNA3.1-msEGFP1.8

    Plasmid
    #208619
    Purpose
    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.
    Depositor
    Quanfu Ma
    Insert
    msEGFP1.8
    Expression
    Mammalian
    Mutation
    (compared with pEGFP-C1, protein level) S30R, Y39…
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208619-plasmid-map-sequence-id-418315

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  12. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

    Enlarge

  13. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  15. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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Showing: 461 - 480 of 1350 results